NF-kappa B is induced in the nuclei of cultured rat aortic smooth muscle cells by stimulation of various growth factors

被引：78

作者：

Obata, H ^{[1
]}

Biro, S ^{[1
]}

Arima, N ^{[1
]}

Kaieda, H ^{[1
]}

Kihara, T ^{[1
]}

Eto, H ^{[1
]}

Miyata, M ^{[1
]}

Tanaka, H ^{[1
]}

机构：

[1] KAGOSHIMA UNIV,FAC SCI,DEPT INTERNAL MED 1,KAGOSHIMA 890,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1996年 / 224卷 / 01期

关键词：

D O I：

10.1006/bbrc.1996.0979

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We investigated whether induction of transcription factor NF-kappa B is involved in the proliferation of cultured rat aortic smooth muscle cell using electrophoretic mobility shift assay and immunocytochemistry. NF-kappa B was induced in the nucleus in a dose-dependent manner when the smooth muscle cells were stimulated by various growth factors such as PDGF-BB, bFGF, EGF and IGF-1, but not growth inhibitors such as TGF-beta and IFN-gamma. Among growth factors, PDGF-BB and bFGF, more potent growth stimulators, induced higher kappa B binding activity than EGF or IGF-1. These evidences were also supported by the results obtained with immunocytochemistry. Immunocytochemistry also showed that the induced NF-kappa B contained p50 and p65. These results suggest that NF-kappa B induction may be involved in the proliferation of vascular smooth muscle cell. (C) 1996 Academic Press, Inc.

引用

页码：27 / 32

页数：6

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