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Subunit-specific temporal and spatial patterns of AMPA receptor exocytosis in hippocampal neurons
被引:511
作者:
Passafaro, M
Piëch, V
Sheng, M
机构:
[1] Massachusetts Gen Hosp, Dept Neurobiol, Boston, MA 02114 USA
[2] Massachusetts Gen Hosp, Howard Hughes Med Inst, Boston, MA 02114 USA
[3] Harvard Univ, Sch Med, Boston, MA 02114 USA
关键词:
D O I:
10.1038/nn0901-917
中图分类号:
Q189 [神经科学];
学科分类号:
071006 ;
摘要:
Using a thrombin cleavage assay in cultured hippocampal neurons, we studied the kinetics, regulation and site of AMPA receptor surface delivery. Surface insertion of the GluR1 subunit occurs slowly in basal conditions and is stimulated by NMDA receptor activation and insulin, whereas GluR2 exocytosis is constitutively rapid. Although both subunits ultimately concentrate in synapses, GluR1 and GluR2 show different spatial patterns of surface accumulation, consistent with GluR1 being inserted initially at extrasynaptic sites and GluR2 being inserted more directly at synapses. The spatiotemporal pattern of surface accumulation is determined by the cytoplasmic tails of GluR subunits, and in heteromeric receptors, GluR1 acts dominantly over GluR2. We propose that GluR1 controls the exocytosis and GluR2/3, the recycling and endocytosis of AMPA receptors.
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页码:917 / 926
页数:10
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