A copper cofactor for the ethylene receptor ETR1 from Arabidopsis

被引:496
作者
Rodriguez, FI [1 ]
Esch, JJ [1 ]
Hall, AE [1 ]
Binder, BM [1 ]
Schaller, GE [1 ]
Bleecker, AB [1 ]
机构
[1] Univ Wisconsin, Dept Bot, Madison, WI 53706 USA
关键词
D O I
10.1126/science.283.5404.996
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The ETR1 receptor from Arabidopsis binds the gaseous hormone ethylene, A copper ion associated with the ethylene-binding domain is required for high-affinity ethylene-binding activity. A missense mutation in the domain that renders the plant insensitive to ethylene eliminates both ethylene binding and the interaction of topper with the receptor. A sequence from the genome of the cyanobacterium Synechocystis sp, strain 6803 that shows homology to the ethylene-binding domain of ETR1 encodes a functional ethylene-binding protein. On the basis of sequence conservation between the Arabidopsis and the cyanobacterial ethylene-binding domains and on in vitro mutagenesis of ETR1, a structural model for this copper-based ethylene sensor domain is presented.
引用
收藏
页码:996 / 998
页数:3
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