Gastrin stimulates the formation of a p60Src/p125FAK complex upstream of the phosphatidylinositol 3-kinase signaling pathway

被引：35

作者：

Daulhac, L ^{[1
]}

Kowalski-Chauvel, A ^{[1
]}

Pradayrol, L ^{[1
]}

Vaysse, N ^{[1
]}

Seva, C ^{[1
]}

机构：

[1] CHU Rangueil, Inst Louis Bugnard, Grp Rech Biol & Pathol Digest, INSERM,U151, F-31403 Toulouse, France

来源：

FEBS LETTERS | 1999年 / 445卷 / 2-3期

关键词：

gastrin; G protein-coupled receptor; phosphatidylinositol; 3-kinase; Src-family kinases; focal adhesion kinase; cellular proliferation;

D O I：

10.1016/S0014-5793(99)00129-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The molecular events whereby gastrin occupancy of G/CCKB receptors leads to phosphatidylinositol (PI) 3-kinase activation have been examined, We report here that this peptide promotes the association between two non-receptor tyrosine kinases, p60(Src) and p125(FAK), and elicits a parallel increase in tyrosine phosphorylation and activity of both kinases. Gastrin-induced PI 3-kinase activity was coprecipitated with p60(Src) and p125(FAK) and was inhibited by herbimycin A, the selective Src inhibitor PP-2 or cytochalasin D, which disrupts the actin cytoskeleton and prevents p125(FAK) activity. These results indicate, for the first time, that a p60(Src)/p125(FAK) complex acts upstream of the gastrin-stimulated PI 3-kinase pathway, (C) 1999 Federation of European Biochemical Societies.

引用

页码：251 / 255

页数：5

共 37 条

[11] PHOSPHORYLATION SITES IN THE PDGF RECEPTOR WITH DIFFERENT SPECIFICITIES FOR BINDING GAP AND PI3 KINASE INVIVO
KASHISHIAN, A
KAZLAUSKAS, A
COOPER, JA
[J]. EMBO JOURNAL, 1992, 11 (04) : 1373 - 1382
[12] Gastrin stimulates tyrosine phosphorylation of insulin receptor substrate 1 and its association with Grb2 and the phosphatidylinositol 3-kinase
KowalskiChauvel, A
Pradayrol, L
Vaysse, N
Seva, C
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (42) : 26356 - 26361
[13] KUSYK CJ, 1986, AM J PHYSIOL, V251, P597
[14] PROTEIN-TYROSINE KINASE PYK2 INVOLVED IN CA2+-INDUCED REGULATION OF ION-CHANNEL AND MAP KINASE FUNCTIONS
LEV, S
MORENO, H
MARTINEZ, R
CANOLL, P
PELES, E
MUSACCHIO, JM
PLOWMAN, GD
RUDY, B
SCHLESSINGER, J
[J]. NATURE, 1995, 376 (6543) : 737 - 745
[15] G protein-coupled receptors mediate two functionally distinct pathways of tyrosine phosphorylation in rat 1a fibroblasts - Shc phosphorylation and receptor endocytosis correlate with activation of Erk kinases
Luttrell, LM
Daaka, Y
DellaRocca, GJ
Lefkowitz, RJ
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (50) : 31648 - 31656
[16] Role of c-Src tyrosine kinase in G protein-coupled receptor- and G beta gamma subunit-mediated activation of mitogen-activated protein kinases
Luttrell, LM
Hawes, BE
vanBiesen, T
Luttrell, DK
Lansing, TJ
Lefkowitz, RJ
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (32) : 19443 - 19450
[17] Involvement of protein kinase C and Src family tyrosine kinase in Gαq/11-induced activation of c-jun N-terminal kinase and p38 mitogen-activated protein kinase
Nagao, M
Yamauchi, J
Kaziro, Y
Itoh, H
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (36) : 22892 - 22898
[18] G-PROTEIN-COUPLED CHEMOATTRACTANT RECEPTORS REGULATE LYN TYROSINE KINASE-CENTER-DOT-SHC ADAPTER PROTEIN SIGNALING COMPLEXES
PTASZNIK, A
TRAYNORKAPLAN, A
BOKOCH, GM
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (34) : 19969 - 19973
[19] RANKIN S, 1994, J BIOL CHEM, V269, P704
[20] THE SMALL GTP-BINDING PROTEIN RHO REGULATES THE ASSEMBLY OF FOCAL ADHESIONS AND ACTIN STRESS FIBERS IN RESPONSE TO GROWTH-FACTORS
RIDLEY, AJ
HALL, A
[J]. CELL, 1992, 70 (03) : 389 - 399

← 1 2 3 4 →