Direct determination of diuretic drugs in urine by capillary zone electrophoresis using fluorescence detection

被引：44

作者：

Gonzalez, E ^{[1
]}

Becerra, A ^{[1
]}

Laserna, JJ ^{[1
]}

机构：

[1] UNIV MALAGA,DEPT ANALYT CHEM,E-29071 MALAGA,SPAIN

来源：

JOURNAL OF CHROMATOGRAPHY B | 1996年 / 687卷 / 01期

关键词：

amiloride; triamterene; bendroflumethiazide; bumetanide;

D O I：

10.1016/S0378-4347(96)00100-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Four diuretic drugs banned in sport (amiloride, triamterene, bendroflumethiazide and bumetanide) have been separated by capillary zone electrophoresis (CZE) and detected using conventional fluorescence spectrometry. The effect of pH on electrophoretic parameters such as migration time, peak efficiency and peak height is discussed. Complete separation of the four drugs is achieved in less than 8 min at pH 8. No interference due to endogenous urine components is observed and thus direct urine analysis is feasible. Analytical figures of merit including precision and limits of detection are presented. Limits of detection range between 0.5 fmol for triamterene and 21.6 fmol for bumetanide.

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页码：145 / 150

页数：6

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