ENANTIOMERIC DIFFERENTIATION OF A WIDE-RANGE OF PHARMACOLOGICALLY ACTIVE SUBSTANCES BY CAPILLARY ELECTROPHORESIS USING MODIFIED BETA-CYCLODEXTRINS

This paper shows the versatility of modified charged and uncharged beta-cyclodextrins (CDs) in the direct chiral resolution of beta-agonists, beta-antagonists, phenylethyamines and alcohol stimulants, and thalidomide and its metabolites. A total of 42 compounds were optically resolved using hydroxypropyl-beta-CD and 20 with sodium sulfobutyl ether-beta-CD. The degree of enantiomeric separation for most substances is dependent on the modified CD concentration. The separation efficiency reaches a maximum at a particular CD concentration. The separation efficiency reaches a maximum at a particular CD concentration, after which further increases in CD concentration causes a progressive decrease in chiral differentiation. Chiral separation of amphetamine enantiomers indicated that a three-point hydrogen bond interaction between the chiral guest molecule and host CD is not necessary for chiral separation under the conditions used.