mAtNOS1 regulates mitochondrial functions and apoptosis of human neuroblastoma cells

被引：19

作者：

Parihar, Mordhwaj S.

Parihar, Arti

Chen, Zhonghai

Nazarewicz, Rafal

Ghafourifar, Pedram ^{[1
]}

机构：

[1] Ohio State Univ, Dept Surg, Davis Heart & Lung Res Inst, Columbus, OH USA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 2008年 / 1780卷 / 06期

关键词：

SHSY; mitochondria; nitric oxide; transmembrane potential; apoptosis;

D O I：

10.1016/j.bbagen.2008.02.006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

mAtNOS1 is a novel gene recently reported in mammalian cells with functions that are not fully understood. The present study generated human neuroblastoma SHSY cells over- and underexpressing mAtNOS1 and shows that mAtNOS1 is involved in regulating mitochondrial nitric oxide, mitochondrial transmembrane potential, protein tyrosine nitration, cytochrome c release, and apoptosis of those cells. (c) 2008 Elsevier B.V. All rights reserved.

引用

收藏

页码：921 / 926

页数：6

相关论文

共 23 条

[1] Nitric oxide mediates glutamate induced mitochondrial depolarization in rat cortical neurons [J].

Almeida, A ;

Bolaños, JP ;

Medina, JM .

BRAIN RESEARCH, 1999, 816 (02) :580-586

[2] Oxidative damage and tyrosine nitration from peroxynitrite [J].

Beckman, JS .

CHEMICAL RESEARCH IN TOXICOLOGY, 1996, 9 (05) :836-844

[3] Mechanisms for nitric oxide synthesis in plants [J].

Crawford, NM .

JOURNAL OF EXPERIMENTAL BOTANY, 2006, 57 (03) :471-478

[4] Modulation of mitochondrial Ca2+ by nitric oxide in cultured bovine vascular endothelial cells [J].

Dedkova, EN ;

Blatter, LA .

AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2005, 289 (04) :C836-C845

[5] Intracellular fluorescent probe concentrations by confocal microscopy [J].

Fink, C ;

Morgan, F ;

Loew, LM .

BIOPHYSICAL JOURNAL, 1998, 75 (04) :1648-1658

[6] Determination of mitochondrial nitric oxide synthase activity [J].

Ghafourifar, P ;

Asbury, ML ;

Joshi, SS ;

Kincaid, ED .

NITRIC OXIDE, PT E, 2005, 396 :424-444

[7] Mitochondrial nitric-oxide synthase stimulation causes cytochrome c release from isolated mitochondria -: Evidence for intramitochondrial peroxynitrite formation [J].

Ghafourifar, P ;

Schenk, U ;

Klein, SD ;

Richter, C .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (44) :31185-31188

[8] Apoptosis - Death deceiver [J].

Green, DR .

NATURE, 1998, 396 (6712) :629-630

[9] Arabidopsis nitric oxide synthase1 is targeted to mitochondria and protects against oxidative damage and dark-induced senescence [J].

Guo, FQ ;

Crawford, NM .

PLANT CELL, 2005, 17 (12) :3436-3450

[10] Identification of a plant nitric oxide synthase gene involved in hormonal signaling [J].

Guo, FQ ;

Okamoto, M ;

Crawford, NM .

SCIENCE, 2003, 302 (5642) :100-103