Effect of O6-methylguanine on the stability of G-quadruplex

被引：49

作者：

Mekmaysy, Chongkham S. ^{[1
]}

Petraccone, Luigi ^{[1
,2
]}

Garbett, Nichola C. ^{[1
]}

Ragazzon, Patricia A. ^{[1
]}

Gray, Robert ^{[1
]}

Trent, John O. ^{[1
]}

Chaires, Jonathan B. ^{[1
]}

机构：

[1] Univ Louisville, Louisville, KY 40202 USA

[2] Univ Naples Federico II, Dept Chem P Corradini, I-80126 Naples, Italy

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2008年 / 130卷 / 21期

关键词：

D O I：

10.1021/ja801976h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The effects of substitution of O-6-methylguanine on the structure and stability of a human telomere quadruplex was studied by circular dichroism, thermal denaturation, analytical ultracentrifugation, and molecular dynamics simulations. The results show that, while quadruplex structures can form containing the modified base, they are much less stable than the normal unmodified structure. The extent of destabilization is critically dependent on the exact position of the modified base within the quadruplex structure.

引用

收藏

页码：6710 / +

页数：3

相关论文

共 19 条

[1] Quadruplex DNA: sequence, topology and structure [J].

Burge, Sarah ;

Parkinson, Gary N. ;

Hazel, Pascale ;

Todd, Alan K. ;

Neidle, Stephen .

NUCLEIC ACIDS RESEARCH, 2006, 34 (19) :5402-5415

[2] Structure of the Hybrid-2 type intramolecular human telomeric G-quadruplex in K+ solution:: insights into structure polymorphism of the human telomeric sequence [J].

Dai, Jixun ;

Carver, Megan ;

Punchihewa, Chandanamali ;

Jones, Roger A. ;

Yang, Danzhou .

NUCLEIC ACIDS RESEARCH, 2007, 35 (15) :4927-4940

[3] NMR solution structure of the major G-quadruplex structure formed in the human BCL2 promoter region [J].

Dai, Jixun ;

Chen, Ding ;

Jones, Roger A. ;

Hurley, Laurence H. ;

Yang, Danzhou .

NUCLEIC ACIDS RESEARCH, 2006, 34 (18) :5133-5144

[4]

DEMPLE B, 1982, J BIOL CHEM, V257, P13776

[5] Chromosomal instability, reproductive cell death and apoptosis induced by O6-methylguanine in Mex-, Mex+ and methylation-tolerant mismatch repair compromised cells:: facts and models [J].

Kaina, B ;

Ziouta, A ;

Ochs, K ;

Coquerelle, T .

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 1997, 381 (02) :227-241

[6]

KOIKE G, 1990, J BIOL CHEM, V265, P14754

[7] Not so crystal clear: the structure of the human telomere G-quadruplex in solution differs from that present in a crystal [J].

Li, J ;

Correia, JJ ;

Wang, L ;

Trent, JO ;

Chaires, JB .

NUCLEIC ACIDS RESEARCH, 2005, 33 (14) :4649-4659

[8] 6-Thioguanine alters the structure and stability of duplex DNA and inhibits quadruplex DNA formation [J].

Marathias, VM ;

Sawicki, MJ ;

Bolton, PH .

NUCLEIC ACIDS RESEARCH, 1999, 27 (14) :2860-2867

[9] G-WIRES - SELF-ASSEMBLY OF A TELOMERIC OLIGONUCLEOTIDE, D(GGGGTTGGGG), INTO LARGE SUPERSTRUCTURES [J].

MARSH, TC ;

HENDERSON, E .

BIOCHEMISTRY, 1994, 33 (35) :10718-10724

[10] A NEW DNA NANOSTRUCTURE, THE G-WIRE, IMAGED BY SCANNING PROBE MICROSCOPY [J].

MARSH, TC ;

VESENKA, J ;

HENDERSON, E .

NUCLEIC ACIDS RESEARCH, 1995, 23 (04) :696-700