Fluorescence in situ hybridization (FISH) on maize metaphase chromosomes with quantum dot-labeled DNA conjugates

被引：39

作者：

Ma, Lu ^{[1
]}

Wu, Sheng-Mei ^{[2
]}

Huang, Jing ^{[1
]}

Ding, Yi ^{[1
]}

Pang, Dai-Wen ^{[2
]}

Li, Lijia ^{[1
]}

机构：

[1] Wuhan Univ, Coll Life Sci, Key Lab MOE Plant Dev Biol, Wuhan 430072, Peoples R China

[2] Wuhan Univ, Coll Chem & Mol Sci, State Key Lab Virol, Wuhan 430072, Peoples R China

来源：

CHROMOSOMA | 2008年 / 117卷 / 02期

基金：

中国国家自然科学基金;

关键词：

D O I：

10.1007/s00412-007-0136-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Semiconductor nanocrystals, also called quantum dots (QDs), are novel inorganic fluorophores which are brighter and more photostable than organic fluorophores. In the present study, highly dispersive QD-labeled oligonucleotide (TAG)(8) (QD-deoxyribonucleic acid [DNA]) conjugates were constructed via the metal-thiol bond, which can be used as fluorescence in situ hybridization (FISH) probes. FISH analysis of maize metaphase chromosomes using the QD-DNA probes showed that the probes could penetrate maize chromosomes and nuclei and solely hybridized to complementary target DNAs. Compared with the conventional organic dyes such as Cy3 and fluorescein isothiocyanate, this class of luminescent labels bound with oligonucleotides is brighter and more stable against photobleaching on the chromosomes after FISH. These results suggest that QD fluorophores may be a more stable and useful fluorescent label for FISH applications in plant chromosome mapping considering their size-tunable luminescence spectra.

引用

页码：181 / 187

页数：7

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