Lipid lateral diffusion in ordered and disordered phases in raft mixtures

被引:123
作者
Filippov, A [1 ]
Orädd, G [1 ]
Lindblom, G [1 ]
机构
[1] Umea Univ, Dept Biophys Chem, SE-90187 Umea, Sweden
关键词
D O I
10.1016/S0006-3495(04)74164-8
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Lipid lateral diffusion coefficients in the quarternary system of dioleoylphosphatidylcholine ( DOPC), sphingomyelin, cholesterol, and water were determined by the pulsed field gradient NMR technique on macroscopically aligned bilayers. The molar ratio between dioleoylphosphatidylcholine and sphingomyelin was set to 1: 1, the cholesterol content was varied between 0 and 45 mol %, the water content was 40 wt %, and the temperature was varied between 293 and 333 K. The diffusion coefficients were separated into fast and slow spectral components by using the CORE method for global analysis of correlated spectral data. A large two-phase region, tentatively assigned to the liquid disordered (l(d)) and the liquid ordered (l(o)) phases, was present in the phase diagram. The ld phase was enriched in dioleoylphosphatidylcholine and exhibited diffusion coefficients that were about three to five times larger than for the l(o) phase. Both the diffusion coefficients and the apparent activation energies for the quarternary systems were compatible with earlier reports on ternary phospholipid/ cholesterol/ water systems. However, in contrast to the latter ternary systems, the exchange of lipids between the lo and the ld phases was slow on the timescale for the diffusion experiment for the quarternary ones. This means that on the millisecond timescale fluid, ordered domains are floating around in a sea of faster diffusing lipids, assigned to consist of mainly dioleoylphosphatidylcholine.
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页码:891 / 896
页数:6
相关论文
共 37 条
[1]   On the origin of sphingolipid/cholesterol-rich detergent-insoluble cell membranes: Physiological concentrations of cholesterol and sphingolipid induce formation of a detergent-insoluble, liquid-ordered lipid phase in model membranes [J].
Ahmed, SN ;
Brown, DA ;
London, E .
BIOCHEMISTRY, 1997, 36 (36) :10944-10953
[2]   LATERAL DIFFUSION IN THE LIQUID-PHASES OF DIMYRISTOYLPHOSPHATIDYLCHOLINE CHOLESTEROL LIPID BILAYERS - A FREE-VOLUME ANALYSIS [J].
ALMEIDA, PFF ;
VAZ, WLC ;
THOMPSON, TE .
BIOCHEMISTRY, 1992, 31 (29) :6739-6747
[3]  
Brown RE, 1998, J CELL SCI, V111, P1
[4]   Lipid rafts reconstituted in model membranes [J].
Dietrich, C ;
Bagatolli, LA ;
Volovyk, ZN ;
Thompson, NL ;
Levi, M ;
Jacobson, K ;
Gratton, E .
BIOPHYSICAL JOURNAL, 2001, 80 (03) :1417-1428
[5]   LIPID AND WATER DIFFUSION IN BICONTINUOUS CUBIC PHASES MEASURED BY NMR [J].
ERIKSSON, PO ;
LINDBLOM, G .
BIOPHYSICAL JOURNAL, 1993, 64 (01) :129-136
[6]   Influence of cholesterol and water content on phospholipid lateral diffusion in bilayers [J].
Filippov, A ;
Orädd, G ;
Lindblom, G .
LANGMUIR, 2003, 19 (16) :6397-6400
[7]   The effect of cholesterol on the lateral diffusion of phospholipids in oriented bilayers [J].
Filippov, A ;
Orädd, G ;
Lindblom, G .
BIOPHYSICAL JOURNAL, 2003, 84 (05) :3079-3086
[8]   Structure, composition, and peptide binding properties of detergent soluble bilayers and detergent, resistant rafts [J].
Gandhavadi, M ;
Allende, D ;
Vidal, A ;
Simon, SA ;
McIntosh, TJ .
BIOPHYSICAL JOURNAL, 2002, 82 (03) :1469-1482
[9]   Influence of cholesterol on the biophysical properties of the sphingomyelin/DOPC binary system [J].
Hao, YH ;
Chen, JW .
JOURNAL OF MEMBRANE BIOLOGY, 2001, 183 (02) :85-92
[10]  
IPSEN JH, 1987, BIOCHIM BIOPHYS ACTA, V905, P162