Site-specific incorporation of methyl- and acetyl-lysine analogues into recombinant proteins

被引：102

作者：

Guo, Jiantao ^{[1
,2
]}

Wang, Jiangyun ^{[1
,2
]}

Lee, Jong Seok ^{[1
,2
]}

Schultz, Peter G. ^{[1
,2
]}

机构：

[1] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

[2] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2008年 / 47卷 / 34期

关键词：

dehydroalanine; histones; lysine derivatives; protein modifications;

D O I：

10.1002/anie.200802336

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Chemical Equation Presented) Location, location, location: A new chemoselective method to site- and degree-specifically install lysine analogues into recombinant proteins provides facile access to near-homogeneously methylated and acetylated histone analogues (see scheme; R1, R 2, R3 = H, Me, acetyl), which can be used to study the impact of histone modification on chromatin structure and function. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：6399 / 6401

页数：3

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