Interaction between estradiol and cAMP in the regulation of specific gene expression

被引：22

作者：

ElTanani, MKK ^{[1
]}

Green, CD ^{[1
]}

机构：

[1] UNIV LIVERPOOL, DEPT BIOCHEM, LIVERPOOL L69 3BX, MERSEYSIDE, ENGLAND

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 1996年 / 124卷 / 1-2期

关键词：

estradiol; cAMP; gene expression;

D O I：

10.1016/S0303-7207(96)03930-5

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The mRNA levels of LIV-1 and pS2, two estrogen-responsive genes, are increased by the agents, cholera toxin (CT) plus 3-isobutyl-1-methylxanthine (IBMX), which cause an increase in cAMP in MCF-7 human-breast cancer cells. The simultaneous addition of estradiol and CT/IBMX results in a synergistic induction of the two mRNAs. The changes in mRNA reflect changes in transcription of the two genes. Interestingly, the addition of CT/IBMX to estradiol not only causes a greater increase in transcription rate but the increase is longer-lasting than that seen with the hormone alone. Stimulation of mRNA levels by CT/IBMX, but not by estradiol, was prevented by cycloheximide. Stimulation by both estradiol and by CT/IBMX was prevented by the antiestrogen, ICI 164387. Transcription of LIV-1 and pS2 genes is regulated by both estradiol and cAMP, via separate mechanisms both requiring the estrogen receptor. Copyright (C) 1996 Elsevier Science Ireland Ltd.

引用

页码：71 / 77

页数：7

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