Non-exponential bleaching of single bioconjugated Cy5 molecules

被引：29

作者：

Füreder-Kitzmüller, E ^{[1
]}

Hesse, J ^{[1
]}

Ebner, A ^{[1
]}

Gruber, HJ ^{[1
]}

Schütz, GJ ^{[1
]}

机构：

[1] Johannes Kepler Univ, Inst Biophys, A-4040 Linz, Austria

来源：

CHEMICAL PHYSICS LETTERS | 2005年 / 404卷 / 1-3期

基金：

奥地利科学基金会;

关键词：

D O I：

10.1016/j.cplett.2005.01.053

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Photobleaching behavior of Cy5-conjugated bovine serum albumin (BSA) was investigated on the ensemble and single molecule level. For ensemble studies, a model was developed which accounts for different excitation rates of randomly oriented dye molecules. Both ensemble and single molecule observations yield non-exponential photobleaching dynamics of BSA-Cy5, indicating the existence of multiple bleaching pathways. (C) 2005 Elsevier B.V. All rights reserved.

引用

页码：13 / 18

页数：6

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