Patterning of photosensitive polyimide LB film and its application in the fabrication of biomolecular microphotodiode array

被引：25

作者：

Oh, SY ^{[1
]}

Park, JK ^{[1
]}

Ko, CB ^{[1
]}

Choi, JW ^{[1
]}

机构：

[1] Sogang Univ, Dept Chem Engn, Seoul 121742, South Korea

来源：

BIOSENSORS & BIOELECTRONICS | 2003年 / 19卷 / 02期

关键词：

polyimide LB film; micro array pattern; cytochrome c; GFP; self-assembly process; redox activity; photodiode;

D O I：

10.1016/S0956-5663(03)00177-5

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Ultra thin film of photosensitive polyimide having benzene and sulfonyloxyimide moieties in the main chain was prepared using a Langmuir-Blodgett (LB) technique, and then micro array pattern of the polyimide LB film on a gold substrate was obtained by deep UV lithographic technique. In order to array cytochrome c molecules along the micro-patterned gold substrate, the well-characterized monolayer of cytochrome c was immobilized with a mixed monolayer of 11-mercaptoundecanoic acid (11-MUDA) and decanethiol. The redox activity and electron transfer between cytochrome c molecular center and gold electrode interface for the self-assembled cytochrome c monolayer were investigated by cyclic voltammetry measurement. Biomolecular photodiode consisting of cytochrome c and green fluorescent protein (GFP) onto the patterned gold substrate was fabricated by self-assembly process. The integration and morphology of cytochrome c and GFP were studied from the measurements of atomic force microscopy (AFM) and fluorescence emission. Especially, current-voltage characteristics of the protein multilayers were investigated by scanning tunneling microscopy (STM) and its application in biomolecular photodiode was also examined. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：103 / 108

页数：6

共 13 条

[1] Investigation of the electrode reaction of cytochrome c through mixed self-assembled monolayers of alkanethiols on gold(111) surfaces [J].