The impact of storage effects in biobanks on biomarker discovery in systems biology studies

被引：6

作者：

Balasubramanian, Raji ^{[2
]}

Mueller, Laurin ^{[1
]}

Kugler, Karl ^{[1
]}

Hackl, Werner ^{[1
]}

Pleyer, Lisa ^{[3
,4
]}

Dehmer, Matthias ^{[1
]}

Graber, Armin ^{[1
]}

机构：

[1] UMIT, Inst Bioinformat & Translat Res, A-6060 Hall In Tirol, Austria

[2] Univ Massachusetts, Sch Publ Hlth & Hlth Sci, Div Biostat & Epidemiol, Amherst, MA 01003 USA

[3] Paracelsus Med Univ Salzburg, Lab Immunol & Mol Canc Res, Salzburg, Austria

[4] Third Med Dept Hematol Med Oncol Hemostaseol Rheu, Salzburg, Austria

来源：

BIOMARKERS | 2010年 / 15卷 / 08期

关键词：

Computational biology; breast cancer; prostate cancer; MASS-SPECTROMETRY; BLOOD COLLECTION; CANCER; TUBES; EXPRESSION; DIAGNOSIS; PATTERNS; DESIGN;

D O I：

10.3109/1354750X.2010.511265

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Sample handling and storage conditions in specimens frozen over long periods of time can severely impact marker levels. If laboratory technologies, practices and related protocols change over time, biomarker studies are potentially biased and report erroneous results. These issues and pitfalls are often overlooked in system biology studies using previously collected and stored materials, and are likely to be one notable cause for biomarker candidates failing to be validated. We present results from simulation studies quantifying the loss in statistical power to detect true biomarkers, due to diminishing concentration of analytes in samples subject to poor handling and storage conditions.</.

引用

页码：677 / 683

页数：7

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