Cytotoxicity of δ-tocotrienols from Kielmeyera coriacea against cancer cell lines

被引：29

作者：

de Mesquita, Mariana Laundry ^{[1
]}

Araujo, Renata Mendonca ^{[2
]}

Bezerra, Daniel Pereira ^{[3
]}

Braz Filho, Raimundo ^{[4
]}

de Paula, Jose Elias ^{[5
]}

Silveira, Edilberto Rocha ^{[2
]}

Pessoa, Claudia ^{[3
]}

de Moraes, Manoel Odorico ^{[3
]}

Costa Lotufo, Leticia Veras ^{[3
]}

Espindola, Laila Salmen ^{[1
]}

机构：

[1] Univ Brasilia, Lab Farmacognosia, Brasilia, DF, Brazil

[2] Univ Fed Ceara, Dept Quim Organ & Inorgan, Fortaleza, Ceara, Brazil

[3] Univ Fed Ceara, Dept Fisiol & Farmacol, Fortaleza, Ceara, Brazil

[4] FAPERJ UENF UFRRJ, Rio De Janeiro, Brazil

[5] Univ Brasilia, Lab Anat Vegetal, Brasilia, DF, Brazil

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 2011年 / 19卷 / 01期

关键词：

Kielmeyera coriacea; HL-60; DNA synthesis; delta-Tocotrienols; Apoptosis; Necrosis; BRAZILIAN CERRADO; TRADITIONAL MEDICINE; GAMMA-TOCOTRIENOL; RICH FRACTION; IN-VITRO; APOPTOSIS; INHIBITION; GROWTH;

D O I：

10.1016/j.bmc.2010.10.044

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

In the search for new anti-cancer compounds, Brazilian Cerrado plant species have been investigated. The hexane root bark extract of Kielmeyera coriacea lead to a mixture of delta-tocotrienol (1) and its dimer (2). The structures of both compounds 1 and 2 were established based on detailed 1D and 2D NMR and EI-MS analyses. The cytotoxicity of the mixture was tested against four human tumor cell lines in the following cultures: MDA-MB-435 (melanoma), HCT-8 (colon), HL-60 (leukemia), and SF-295 (glioblastoma), and displayed IC50 values ranging from 8.08 to 23.58 mu g/mL. Additional assays were performed in order to investigate the mechanism of action of the mixture (1 + 2) against the human leukemia cell line HL-60. The results suggested that the mixture suppressed leukemia growth and reduced cell survival, triggering both apoptosis and necrosis, depending on the concentration. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：623 / 630

页数：8

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