Keratocyte phenotype mediates proteoglycan structure - A role for fibroblasts in corneal fibrosis

被引:199
作者
Funderburgh, JL [1 ]
Mann, MM [1 ]
Funderburgh, ML [1 ]
机构
[1] Univ Pittsburgh, Eye & Ear Inst 1011, Dept Ophthalmol, Pittsburgh, PA 15213 USA
关键词
D O I
10.1074/jbc.M303292200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In pathological corneas, accumulation of fibrotic extracellular matrix is characterized by proteoglycans with altered glycosaminoglycans that contribute to the reduced transparency of scarred tissue. During wound healing, keratocytes in the corneal stroma transdifferentiate into fibroblasts and myofibroblasts. In this study, molecular markers were developed to identify keratocyte, fibroblast, and myofibroblast phenotypes in primary cultures of corneal stromal cells and the structure of glycosaminoglycans secreted by these cells was characterized. Quiescent primary keratocytes expressed abundant protein and mRNA for keratocan and aldehyde dehydrogenase class 3 and secreted proteoglycans containing macromolecular keratan sulfate. Expression of these marker compounds was reduced in fibroblasts and also in transforming growth factor-beta-induced myofibroblasts, which expressed high levels of alpha-smooth muscle actin, biglycan, and the extra domain A (EDA or EIIIA) form of cellular fibronectin. Collagen types I and III mRNAs were elevated in both fibroblasts and in myofibroblasts. Expression of these molecular markers clearly distinguishes the phenotypic states of stromal cells in vitro. Glycosaminoglycans secreted by fibroblasts and myofibroblasts were qualitatively similar to and differed from those of keratocytes. Chondroitin/dermatan sulfate abundance, chain length, and sulfation were increased as keratocytes became fibroblasts and myofibroblasts. Fluorophore-assisted carbohydrate electrophoresis analysis demonstrated increased N-acetylgalactosamine sulfation at both 4- and 6-carbons. Hyaluronan, absent in keratocytes, was secreted by fibroblasts and myofibroblasts. Keratan sulfate biosynthesis, chain length, and sulfation were significantly reduced in both fibroblasts and myofibroblasts. The qualitatively similar expression of glycosaminoglycans shared by fibroblasts and myofibroblasts suggests a role for fibroblasts in deposition of non-transparent fibrotic tissue in pathological corneas.
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收藏
页码:45629 / 45637
页数:9
相关论文
共 66 条
[41]  
Latvala T, 1996, J REFRACT SURG, V12, P677
[42]   Fibroblast growth factor-2 promotes keratan sulfate proteoglycan expression by keratocytes in vitro [J].
Long, CJ ;
Roth, MR ;
Tasheva, ES ;
Funderburgh, M ;
Smit, R ;
Conrad, GW ;
Funderburgh, JL .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (18) :13918-13923
[43]   Myofibroblasts differentiate from fibroblasts when plated at low density [J].
Masur, SK ;
Dewal, HS ;
Dinh, TT ;
Erenburg, I ;
Petridou, S .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (09) :4219-4223
[44]   THE ANTIGENIC DETERMINANTS RECOGNIZED BY 3 MONOCLONAL-ANTIBODIES TO KERATAN SULFATE INVOLVE SULFATED HEPTA-OLIGOSACCHARIDES OR LARGER OLIGOSACCHARIDES OF THE POLY(N-ACETYLLACTOSAMINE) SERIES [J].
MEHMET, H ;
SCUDDER, P ;
TANG, PW ;
HOUNSELL, EF ;
CATERSON, B ;
FEIZI, T .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1986, 157 (02) :385-391
[45]   Glycosyltransferase and sulfotransferase activities in chick corneal stromal cells before and after in vitro culture [J].
Nakazawa, K ;
Takahashi, I ;
Yamamoto, Y .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1998, 359 (02) :269-282
[46]  
NAKAZAWA K, 1984, J BIOL CHEM, V259, P3751
[47]  
Pérez-Santonja JJ, 1998, J REFRACT SURG, V14, P602
[48]   Review: A case for corneal crystallins [J].
Piatigorsky, J .
JOURNAL OF OCULAR PHARMACOLOGY AND THERAPEUTICS, 2000, 16 (02) :173-180
[49]   Altered fine structures of corneal and skeletal keratan sulfate and chondroitin/dermatan sulfate in macular corneal dystrophy [J].
Plaas, AH ;
West, LA ;
Thonar, EJA ;
Karcioglu, ZA ;
Smith, CJ ;
Klintworth, GK ;
Hascall, VC .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (43) :39788-39796
[50]   Keratan sulfate disaccharide composition determined by FACE analysis of keratanase II and endo-β-galactosidase digestion products [J].
Plaas, AHK ;
West, LA ;
Midura, RJ .
GLYCOBIOLOGY, 2001, 11 (10) :779-790