Haematopoiesis in the era of advanced single-cell technologies

被引：97

作者：

Jacobsen, Sten Eirik W. ^{[1
,2
,3
,4
,5
]}

Nerlov, Claus ^{[1
]}

机构：

[1] Univ Oxford, John Radcliffe Hosp, MRC Mol Haematol Unit, MRC Weatherall Inst Mol Med, Oxford, England

[2] Univ Oxford, John Radcliffe Hosp, Haematopoiet Stem Cell Lab, MRC Weatherall Inst Mol Med, Oxford, England

[3] Karolinska Inst, Dept Cell & Mol Biol, Wallenberg Inst Regenerat Med, Stockholm, Sweden

[4] Karolinska Inst, Dept Med Huddinge, Ctr Hematol & Regenerat Med, Stockholm, Sweden

[5] Karolinska Univ Hosp, Stockholm, Sweden

来源：

NATURE CELL BIOLOGY | 2019年 / 21卷 / 01期

基金：

英国生物技术与生命科学研究理事会; 瑞典研究理事会;

关键词：

STEM-CELLS; MYELOID PROGENITOR; SELF-RENEWAL; DIFFERENTIATION PROGRAMS; CLONAL DYNAMICS; GENE-EXPRESSION; REVEALS; MAP; TRANSPLANTATION; RECONSTITUTION;

D O I：

10.1038/s41556-018-0227-8

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

The molecular and functional characterization of single cells at scale has emerged as a key driver to unravel tissue biology. Thus, it is important to understand the strengths and limitations of transcriptomic approaches, molecular barcoding and functional assays used to study cellular properties at the single-cell level. Here, we review recent relevant work from the haematopoietic system and discuss how to interpret and integrate data obtained with different technologies.

引用

收藏

页码：2 / 8

页数：7

相关论文

共 56 条

[1]

Identification of Flt3+ lympho-myeloid stem cells lacking erythro-megakaryocytic potential:: A revised road map for adult blood lineage commitment [J].

Adolfsson, J ;

Månsson, R ;

Buza-Vidas, N ;

Hultquist, A ;

Liuba, K ;

Jensen, CT ;

Bryder, D ;

Yang, LP ;

Borge, OJ ;

Thoren, LAM ;

Anderson, K ;

Sitnicka, E ;

Sasaki, Y ;

Sigvardsson, M ;

Jacobsen, SEW .

CELL, 2005, 121 (02) :295-306

[2]

A clonogenic common myeloid progenitor that gives rise to all myeloid lineages [J].

Akashi, K ;

Traver, D ;

Miyamoto, T ;

Weissman, IL .

NATURE, 2000, 404 (6774) :193-197

[3]

Reciprocal activation of GATA-1 and PU.1 marks initial specification of hematopoietic stem cells into myeloerythroid and myelolymphoid lineages [J].

Arinobu, Yojiro ;

Mizuno, Shin-ichi ;

Chong, Yong ;

Shigematsu, Hirokazu ;

Lino, Tadafumi ;

Iwasaki, Hiromi ;

Graf, Thomas ;

Mayfield, Robin ;

Chan, Susan ;

Kastner, Philippe ;

Akashi, Koichi .

CELL STEM CELL, 2007, 1 (04) :416-427

[4]

Hematopoietic Stem Cells Count and Remember Self-Renewal Divisions [J].

Bernitz, Jeffrey M. ;

Kim, Huen Suk ;

MacArthur, Ben ;

Sieburg, Hans ;

Moore, Kateri .

CELL, 2016, 167 (05) :1296-+

[5]

In Vivo Tracking of Human Hematopoiesis Reveals Patterns of Clonal Dynamics during Early and Steady-State Reconstitution Phases [J].

Biasco, Luca ;

Pellin, Danilo ;

Scala, Serena ;

Dionisio, Francesca ;

Basso-Ricci, Luca ;

Leonardelli, Lorena ;

Scaramuzza, Samantha ;

Baricordi, Cristina ;

Ferrua, Francesca ;

Cicalese, Maria Pia ;

Giannelli, Stefania ;

Neduva, Victor ;

Dow, David J. ;

Schmidt, Manfred ;

Von Kalle, Christof ;

Roncarolo, Maria Grazia ;

Ciceri, Fabio ;

Vicard, Paola ;

Wit, Ernst ;

Di Serio, Clelia ;

Naldini, Luigi ;

Aiuti, Alessandro .

CELL STEM CELL, 2016, 19 (01) :107-119

[6]

SALVAGE TRANSPLANTATION FOR ALLOGRAFT FAILURE UTILIZING FLUDARABINE AND ALEMTUZUMAB AS THE CONDITIONING REGIMEN [J].

Bolanos-Meade, J. ;

Luznik, L. ;

Jones, R. J. ;

Fucbs, E. J. .

BIOLOGY OF BLOOD AND MARROW TRANSPLANTATION, 2008, 14 (02) :110-110

[7]

Integrated Single-Cell Analysis Maps the Continuous Regulatory Landscape of Human Hematopoietic Differentiation [J].

Buenrostro, Jason D. ;

Corces, M. Ryan ;

Lareau, Caleb A. ;

Wu, Beijing ;

Schep, Alicia N. ;

Aryee, Martin J. ;

Majeti, Ravindra ;

Chang, Howard Y. ;

Greenleaf, William J. .

CELL, 2018, 173 (06) :1535-+

[8]

Fundamental properties of unperturbed haematopoiesis from stem cells in vivo [J].

Busch, Katrin ;

Klapproth, Kay ;

Barile, Melania ;

Flossdorf, Michael ;

Holland-Letz, Tim ;

Schlenner, Susan M. ;

Reth, Michael ;

Hoefer, Thomas ;

Rodewald, Hans-Reimer .

NATURE, 2015, 518 (7540) :542-546

[9]

Hierarchically related lineage-restricted fates of multipotent haematopoietic stem cells [J].

Carrelha, Joana ;

Meng, Yiran ;

Kettyle, Laura M. ;

Luis, Tiago C. ;

Norfo, Ruggiero ;

Alcolea, Veronica ;

Boukarabila, Hanane ;

Grasso, Francesca ;

Gambardella, Adriana ;

Grover, Amit ;

Hogstrand, Kari ;

Lord, Allegra M. ;

Sanjuan-Pla, Alejandra ;

Woll, Petter S. ;

Nerlov, Claus ;

Jacobsen, Sten Eirik W. .

NATURE, 2018, 554 (7690) :106-+

[10]

Harnessing transposons for cancer gene discovery [J].

Copeland, Neal G. ;

Jenkins, Nancy A. .

NATURE REVIEWS CANCER, 2010, 10 (10) :696-706

← 1 2 3 4 5 6 →