Comprehensive step-by-step engineering of an (R)-hydroxynitrile lyase for large-scale asymmetric synthesis

被引：84

作者：

Glieder, A

Weis, R

Skranc, W

Poechlauer, P

Dreveny, I

Majer, S

Wubbolts, M

Schwab, H

Gruber, K

机构：

[1] Graz Univ Technol, Inst Biotechnol, A-8010 Graz, Austria

[2] Res Ctr Appl Biocatalysis, A-8010 Graz, Austria

[3] Karl Franzens Univ Graz, Inst Chem, A-8010 Graz, Austria

[4] DSM Fine Chem Austria Nfg GmbH & Co KG, R&D Ctr Linz, A-4021 Linz, Austria

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2003年 / 42卷 / 39期

关键词：

asymmetric synthesis; C-C coupling; enzyme catalysis; protein design; protein engineering;

D O I：

10.1002/anie.200352141

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A custom-made enzyme: The gene and protein sequence of a new (R)-hydroxynitrile lyase from Prunus amygdalus was cloned and engineered for recombinant production on a large scale. The enzyme is stable and active at low pH values. A rationally designed active-site mutant now permits the rapid and stereoselective synthesis of sterically hindered cyanohydrins such as ortho-chloromandelonitrile (see picture).

引用

页码：4815 / 4818

页数：4

共 25 条

[1] D-HYDROXYNITRILE LYASE - INVOLVEMENT OF PROSTHETIC FLAVIN ADENINE-DINUCLEOTIDE IN ENZYME-ACTIVITY [J].