Analysis of the fecal microbiota of irritable bowel syndrome patients and healthy controls with real-time PCR

被引:524
作者
Malinen, E
Rinttilä, T
Kajander, K
Mättö, J
Kassinen, A
Krogius, L
Saarela, M
Korpela, R
Palva, A
机构
[1] Univ Helsinki, Dept Vet Basic Sci, Fac Med Vet, Microbiol Sect, FIN-00014 Helsinki, Finland
[2] Valio Ltd R & D, FIN-00039 Helsinki, Finland
[3] VTT Biotechnol, FIN-02044 Espoo, Finland
[4] Univ Helsinki, Biomembrane Inst, FIN-00014 Helsinki, Finland
关键词
D O I
10.1111/j.1572-0241.2005.40312.x
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
OBJECTIVE: The gut microbiota may contribute to the onset and maintenance of irritable bowel syndrome (IBS). In this study, the microbiotas of patients suffering from IBS were compared with a control group devoid of gastrointestinal (GI) symptoms. METHODS: Fecal microbiota of patients (n = 27) fulfilling the Rome II criteria for IBS, was compared with age- and gender-matched control subjects (n = 22). Fecal samples were obtained at 3 months intervals. Total bacterial DNA was analyzed by 20 quantitative real-time PCR assays covering approximately 300 bacterial species. RESULTS: Extensive individual variation was observed in the GI microbiota among both the IBS- and control groups. Sorting of the IBS patients according to the symptom subtypes (diarrhea, constipation, and alternating predominant type) revealed that lower amounts of Lactobacillus spp. were present in the samples of diarrhea predominant IBS patients wheras constipation predominant IBS, patients carried increased amounts of Veillonella spp. Average results from three fecal samples suggested differences in the Clostridium coccoides; subgroup and Bifidobacterium catenulatum group between IBS patients (n = 21) and controls (n = 15). Of the intestinal pathogens earlier associated with IBS. no indications of Helicobacter spp. or Clostridium difficile were found whereas one case of Campylobacter jejuni was identified by sequencing. CONCLUSIONS: With these real-time PCR assays, quantitative alterations in the GI microbiota of IBS patients were found. Increasing microbial DNA sequence information will further allow designing of new real-time PCR assays for a more extensive analysis of intestinal microbes in IBS.
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页码:373 / 382
页数:10
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