Recombinant human growth/differentiation factor 5 stimulates mesenchyme aggregation and chondrogenesis responsible for the skeletal development of limbs

被引：133

作者：

Hotten, GC ^{[1
]}

Matsumoto, T ^{[1
]}

Kimura, M ^{[1
]}

Bechtold, RF ^{[1
]}

Kron, R ^{[1
]}

Ohara, T ^{[1
]}

Tanaka, H ^{[1
]}

Satoh, Y ^{[1
]}

Okazaki, M ^{[1
]}

Shirai, T ^{[1
]}

Pan, H ^{[1
]}

Kawai, S ^{[1
]}

Pohl, JS ^{[1
]}

Kudo, A ^{[1
]}

机构：

[1] HOECHST JAPAN LTD,PHARMA RES & DEV DIV,DRUG DISCOVERY RES LABS,KAWAGOE,SAITAMA 35011,JAPAN

来源：

GROWTH FACTORS | 1996年 / 13卷 / 1-2期

关键词：

growth/differentiation factor; transforming growth factor-beta superfamily; chondrogenesis; bone formation;

D O I：

10.3109/08977199609034567

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We have expressed and biologically characterized recombinant human growth/differentiation factor 5 (huGDF5). This protein is composed of a mature homodimer consisting of 15 kD subunits. Using recombinant expressed protein, we have demonstrated that huGDF5 in vitro stimulated mesenchyme aggregation and chondrogenesis in rat limb bud cells. In vivo, partially purified huGDF5 induced cartilage and bone formation in muscular tissues of rodents. However, in contrast to the effects of other BMPs, as for example BMP-2, the osteoblastic MC3T3-E1 cells did not respond to huGDF5 as measured by alkaline phosphatase activity. These results suggest that the action of GDF5 may be relatively specific for chondrogenesis during the entire process of the endochondral bone formation. GDF5 may control the morphogenesis of cartilaginous tissue, including joints, in the skeletal development of limbs.

引用

页码：65 / &

页数：12

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