CLONING AND EXPRESSION OF RECOMBINANT HUMAN GROWTH/DIFFERENTIATION FACTOR-5

被引：89

作者：

HOTTEN, G

NEIDHARDT, H

JACOBOWSKY, B

POHL, J

机构：

[1] Biopharm GmbH, 69115 Heidelberg

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 204卷 / 02期

关键词：

D O I：

10.1006/bbrc.1994.2508

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The complete amino acid sequence of human Growth/differentiation factor 5 (huGdf5), a new member of the TGF-beta superfamily, has been determined through initial degenerate PCR and subsequent cloning and nucleotide sequencing of genomic DNA and cDNA endcoding the precursor and flanking regions. The huGdf5 gene consists of only two coding exons. The protein is highly homologous to its murine equivalent, particularly the mature part which differs only by one amino acid. Expression in HuTK(-) cells using recombinant vaccinia virus revealed the expected processed dimeric mature protein. Antibodies against huGdf5 were raised in chicken. (C) 1994 Academic Press, Inc.

引用

页码：646 / 652

页数：7

共 25 条

[1] IDENTIFICATION OF TRANSFORMING GROWTH-FACTOR-BETA FAMILY MEMBERS PRESENT IN BONE-INDUCTIVE PROTEIN PURIFIED FROM BOVINE BONE [J].

CELESTE, AJ ;

IANNAZZI, JA ;

TAYLOR, RC ;

HEWICK, RM ;

ROSEN, V ;

WANG, EA ;

WOZNEY, JM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (24) :9843-9847

[2] TRANSFORMING GROWTH-FACTOR-BETA GENE FAMILY MEMBERS AND BONE [J].

CENTRELLA, M ;

HOROWITZ, MC ;

WOZNEY, JM ;

MCCARTHY, TL .

ENDOCRINE REVIEWS, 1994, 15 (01) :27-39

[3] A NEW TYPE OF TRANSFORMING GROWTH FACTOR-BETA, TGF-BETA-3 [J].

DERYNCK, R ;

LINDQUIST, PB ;

LEE, A ;

WEN, D ;

TAMM, J ;

GRAYCAR, JL ;

RHEE, L ;

MASON, AJ ;

MILLER, DA ;

COFFEY, RJ ;

MOSES, HL ;

CHEN, EY .

EMBO JOURNAL, 1988, 7 (12) :3737-3743

[4] INTRON-EXON STRUCTURE OF THE HUMAN TRANSFORMING GROWTH-FACTOR-BETA PRECURSOR GENE [J].

DERYNCK, R ;

RHEE, L ;

CHEN, EY ;

VANTILBURG, A .

NUCLEIC ACIDS RESEARCH, 1987, 15 (07) :3188-3189

[5] CHROMOSOMAL LOCALIZATION OF 7 MEMBERS OF THE MURINE TGF-BETA SUPERFAMILY SUGGESTS CLOSE LINKAGE TO SEVERAL MORPHOGENETIC MUTANT LOCI [J].

DICKINSON, ME ;

KOBRIN, MS ;

SILAN, CM ;

KINGSLEY, DM ;

JUSTICE, MJ ;

MILLER, DA ;

CECI, JD ;

LOCK, LF ;

LEE, A ;

BUCHBERG, AM ;

SIRACUSA, LD ;

LYONS, KM ;

DERYNCK, R ;

HOGAN, BLM ;

COPELAND, NG ;

JENKINS, NA .

GENOMICS, 1990, 6 (03) :505-520

[6]

EARL PL, 1994, CURRENT PROTOCOLS MO, V2, pCH16

[7]

FROHMAN MA, 1990, AMPLIFICATIONS, V5, P11

[8]

GONELI SA, 1990, BIOCH INT, V21, P685

[9]

HARLOW E, 1988, ANTIBODIES LABORATOR, pCH14

[10] GENETIC APPROACH TO FACILITATE PURIFICATION OF RECOMBINANT PROTEINS WITH A NOVEL METAL CHELATE ADSORBENT [J].

HOCHULI, E ;

BANNWARTH, W ;

DOBELI, H ;

GENTZ, R ;

STUBER, D .

BIO-TECHNOLOGY, 1988, 6 (11) :1321-1325

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