Modifications of Commercial Toxigenic Clostridium difficile PCR Resulting in Improved Economy and Workflow Efficiency

被引：8

作者：

Munson, Erik ^{[1
,2
]}

Bilbo, Dorothy ^{[1
]}

Paul, Mary ^{[1
]}

Napierala, Maureen ^{[1
]}

Hryciuk, Jeanne E. ^{[1
]}

机构：

[1] Wheaton Franciscan Lab, Wauwatosa, WI 53226 USA

[2] Univ Wisconsin, Coll Hlth Sci, Milwaukee, WI 53201 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2011年 / 49卷 / 06期

关键词：

REAL-TIME PCR; STAPHYLOCOCCUS-AUREUS; METHICILLIN-RESISTANT; ENZYME-IMMUNOASSAY; 2-STEP ALGORITHM; ASSAY; INFECTION; DIAGNOSIS; CULTURE; MIX;

D O I：

10.1128/JCM.00261-11

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Expense inherent to molecular diagnostics may prevent laboratories from utilizing real-time PCR for Clostridium difficile infection. Frozen master mix and overnight aliquot modifications of the BD GeneOhm Cdiff assay failed to impact performance indices compared to the package insert protocol ( P >= 0.31), provided accurate results, and decreased reagent expenditure.

引用

页码：2279 / 2282

页数：4

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