Insulin-cell penetrating peptide hybrids with improved intestinal absorption efficiency

被引：104

作者：

Liang, JF ^{[1
]}

Yang, VC

机构：

[1] Stevens Inst Technol, Dept Chem & Biol Chem, Hoboken, NJ 07030 USA

[2] Univ Michigan, Coll Pharm, Ann Arbor, MI 48109 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 335卷 / 03期

关键词：

cell-penetrating peptide; diabetes; oral insulin; Caco-2 cell monolayer; TAT; drug delivery;

D O I：

10.1016/j.bbrc.2005.07.142

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cell-penetrating peptide (CPP) was linked to insulin to form insulin-CPP hybrids. The intestinal absorption efficiency of CPP hybridized insulin was 6-8 times increased compared to normal insulin as tested on Caco-2 cell monolayer, a widely used in vitro model for intestinal absorption. Insulin-CPP hybrid transportation seemed to be through an active and transcytosis-like mechanism. Importantly, insulin in hybrids kept intact after they passed through the Caco-2 cell monolayer. This study provides a new clue for oral insulin development. (C) 2005 Elsevier Inc. All rights reserved.

引用

页码：734 / 738

页数：5

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