Calcium alginate gel as encapsulation matrix for coimmobilized enzyme systems

被引：38

作者：

Blandino, A ^{[1
]}

Macías, A ^{[1
]}

Cantero, D ^{[1
]}

机构：

[1] UCA, Fac Sci, Dept Chem Engn, Biol & Enzymat Reactors Res Grp, Puerto Real 11510, Cadiz, Spain

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2003年 / 110卷 / 01期

关键词：

calcium alginate; gel capsules; gel beads; coimmobilization; glucose oxidase; catalase;

D O I：

10.1385/ABAB:110:1:53

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Encapsulation within calcium alginate gel capsules was used to produce a coimmobilized enzyme system. Glucose oxidase (GOD) and catalase (CAT) were chosen as model enzymes. The same values of V(max) and K(m) (app) for the GOD encapsulated system and for the GOD-CAT coencapsulated system were calculated. When gel beads and capsules were compared, the same catalyst deactivation sequence for the two enzymes was observed. However, when capsules were employed as immobilization support, GOD efficiencies were higher than for the gel beads. These results were explained in terms of the structure of the capsules.

引用

页码：53 / 60

页数：8

共 16 条

[1]

Immobilization of glucose oxidase within calcium alginate gel capsules [J].

Blandino, A ;

Macías, M ;

Cantero, D .

PROCESS BIOCHEMISTRY, 2001, 36 (07) :601-606

[2]

BOLTZ DF, 1987, COLORIMETRIC DETERMI

[3]

SEMIPERMEABLE AQUEOUS MICROCAPSULES .I. PREPARATION AND PROPERTIES [J].

CHANG, TMS ;

MACINTOSH, FC ;

MASON, SG .

CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, 1966, 44 (01) :115-+

[4]

Christenson L., 1993, FUNDAMENTALS ANIMAL, P7

[5]

BIOLOGICAL INTERACTIONS BETWEEN POLYSACCHARIDES AND DIVALENT CATIONS - EGG-BOX MODEL [J].

GRANT, GT ;

MORRIS, ER ;

REES, DA ;

SMITH, PJC ;

THOM, D .

FEBS LETTERS, 1973, 32 (01) :195-198

[6]

C-13-NMR STUDIES OF MONOMERIC COMPOSITION AND SEQUENCE IN ALGINATE [J].

GRASDALEN, H ;

LARSEN, B ;

SMIDSROD, O .

CARBOHYDRATE RESEARCH, 1981, 89 (02) :179-191

[7]

FRACTIONATION OF ALGINIC ACID [J].

HAUG, A .

ACTA CHEMICA SCANDINAVICA, 1959, 13 (03) :601-603

[8]

CHARACTERISTICS OF ALGINATE FROM LAMINARIA-DIGITATA CULTIVATED IN A HIGH-PHOSPHATE ENVIRONMENT [J].

INDERGAARD, M ;

SKJAKBRAEK, G .

HYDROBIOLOGIA, 1987, 151 :541-549

[9]

LINKER A, 1966, J BIOL CHEM, V241, P3845

[10]

ON THE MECHANISM OF IMMOBILIZED GLUCOSE-OXIDASE DEACTIVATION BY HYDROGEN-PEROXIDE [J].

MALIKKIDES, CO ;

WEILAND, RH .

BIOTECHNOLOGY AND BIOENGINEERING, 1982, 24 (11) :2419-2439

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