Hyaluronan Up-Regulates IL-10 Expression in Fibroblast-Like Synoviocytes from Patients with Tibia Plateau Fracture

被引:19
作者
Huang, Teng-Le [1 ,2 ,4 ,5 ]
Hsu, Horng-Chaung [4 ,5 ]
Yang, Kai-Chiang [1 ,2 ]
Lin, Feng-Huei [1 ,2 ,3 ]
机构
[1] Natl Taiwan Univ, Coll Med, Inst Biomed Engn, Taipei 10764, Taiwan
[2] Natl Taiwan Univ, Coll Engn, Taipei 10764, Taiwan
[3] Natl Hlth Res Inst, Div Med Engn, Chun Nan, Taiwan
[4] China Med Univ, Coll Hlth Care, Dept Sports Med, Taichung, Taiwan
[5] Univ Hosp, Taichung, Taiwan
关键词
hyaluronan; tibia plateau fracture; synoviocyte; NF-KAPPA-B; POSTTRANSLATIONAL MODIFICATION; DOWN-REGULATION; OSTEOARTHRITIS; TRANSLATION; INJECTION; CARTILAGE; THERAPY; CELLS; KNEE;
D O I
10.1002/jor.21261
中图分类号
R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学(修复外科学)];
学科分类号
100224 [整形外科学];
摘要
Progression to osteoarthritis (OA) is a frequent sequela of severe articular fracture, particularly when weight-bearingjoints are involved. Prevention from post-traumatic OA remains a challenge. Hyaluronan (HA) therapy is reported to represent a safe and effective treatment for patients with OA and rheumatoid arthritis. However, the capacity of HA to prevent the occurrence of osteoarthritic changes in fractured joints has not been demonstrated. The present study was undertaken to examine the effects of HA on expression of six OA-related proteins in fibroblast-like synoviocytes (FLS) from 10 patients with tibia plateau fracture. OA-related factors were quantified using a sandwich enzyme-linked immunosorbent assay. Regardless of induction of the FLS with interleukin (IL)-1 beta, HA was found to down-regulate expression of catabolic factors (IL-1 beta, matrix metalloproteinase-3, and tumor necrosis factor-alpha) and to up-regulate production of anti catabolic factors (tissue inhibitors of metalloproteinase-1 and metalloproteinase-2). HA also enhanced expression of IL-10, an antiinflammatory cytokine, in FLS. Our results indicated that HA can promote the expression of both antiinflammatory and structure-protective factors in FLS of patients with tibia plateau fracture. (C) 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 29:495-500, 2011
引用
收藏
页码:495 / 500
页数:6
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