Sequence-specific inhibition of microRNA- and siRNA-induced RNA silencing

被引：473

作者：

Meister, G ^{[1
]}

Landthaler, M ^{[1
]}

Dorsett, Y ^{[1
]}

Tuschl, T ^{[1
]}

机构：

[1] Rockefeller Univ, Lab RNA Mol Biol, New York, NY 10021 USA

来源：

RNA | 2004年 / 10卷 / 03期

关键词：

RNA interference; miRNA; miRNP; posttranscriptional gene regulation; translational regulation; mRNA degradation;

D O I：

10.1261/rna.5235104

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A large number of miRNAs have recently been discovered in plants and animals. Development of reverse genetic approaches that act to inhibit microRNA function would facilitate the study of this new class of noncoding RNA. Here we show that 2'-O-methyl oligoribonucleotides, but not 2'-deoxyoligonucleotides specifically inactivate the RNAi activity associated with miRNA-protein complexes in human cell extracts as well as in cultured human cells.

引用

页码：544 / 550

页数：7

共 75 条

[1] The Caenorhabditis elegans hunchback-like gene lin-57/hbl-1 controls developmental time and is regulated by microRNAs [J].

Abrahante, JE ;

Daul, AL ;

Li, M ;

Volk, ML ;

Tennessen, JM ;

Miller, EA ;

Rougvie, AE .

DEVELOPMENTAL CELL, 2003, 4 (05) :625-637

[2] MicroRNA pathways in flies and worms: Growth, death, fat, stress, and timing [J].

Ambros, V .

CELL, 2003, 113 (06) :673-676

[3] MicroRNAs and other tiny endogenous RNAs in C-elegans [J].

Ambros, V ;

Lee, RC ;

Lavanway, A ;

Williams, PT ;

Jewell, D .

CURRENT BIOLOGY, 2003, 13 (10) :807-818

[4] The small RNA profile during Drosophila melanogaster development [J].

Aravin, AA ;

Lagos-Quintana, M ;

Yalcin, A ;

Zavolan, M ;

Marks, D ;

Snyder, B ;

Gaasterland, T ;

Meyer, J ;

Tuschl, T .

DEVELOPMENTAL CELL, 2003, 5 (02) :337-350

[5] Regulation of flowering time and floral organ identity by a microRNA and its APETALA2-like target genes [J].

Aukerman, MJ ;

Sakai, H .

PLANT CELL, 2003, 15 (11) :2730-2741

[6] Developmental defects by antisense-mediated inactivation of micro-RNAs 2 and 13 in Drosophila and the identification of putative target genes [J].

Boutla, A ;

Delidakis, C ;

Tabler, M .

NUCLEIC ACIDS RESEARCH, 2003, 31 (17) :4973-4980

[7] Towards a complete description of the microRNA complement of animal genomes [J].

Brennecke, J ;

Cohen, SM .

GENOME BIOLOGY, 2003, 4 (09)

[8] bantam encodes a developmentally regulated microRNA that controls cell proliferation and regulates the proapoptotic gene hid in Drosophila [J].

Brennecke, J ;

Hipfner, DR ;

Stark, A ;

Russell, RB ;

Cohen, SM .

CELL, 2003, 113 (01) :25-36

[9] Frequent deletions and down-regulation of micro-RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia [J].

Calin, GA ;

Dumitru, CD ;

Shimizu, M ;

Bichi, R ;

Zupo, S ;

Noch, E ;

Aldler, H ;

Rattan, S ;

Keating, M ;

Rai, K ;

Rassenti, L ;

Kipps, T ;

Negrini, M ;

Bullrich, F ;

Croce, CM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (24) :15524-15529

[10] Role of microRNAs in plant and animal development [J].

Carrington, JC ;

Ambros, V .

SCIENCE, 2003, 301 (5631) :336-338

← 1 2 3 4 5 6 7 8 →