Sequence-specific inhibition of microRNA- and siRNA-induced RNA silencing

被引：473

作者：

Meister, G ^{[1
]}

Landthaler, M ^{[1
]}

Dorsett, Y ^{[1
]}

Tuschl, T ^{[1
]}

机构：

[1] Rockefeller Univ, Lab RNA Mol Biol, New York, NY 10021 USA

来源：

RNA | 2004年 / 10卷 / 03期

关键词：

RNA interference; miRNA; miRNP; posttranscriptional gene regulation; translational regulation; mRNA degradation;

D O I：

10.1261/rna.5235104

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A large number of miRNAs have recently been discovered in plants and animals. Development of reverse genetic approaches that act to inhibit microRNA function would facilitate the study of this new class of noncoding RNA. Here we show that 2'-O-methyl oligoribonucleotides, but not 2'-deoxyoligonucleotides specifically inactivate the RNAi activity associated with miRNA-protein complexes in human cell extracts as well as in cultured human cells.

引用

页码：544 / 550

页数：7

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