Bacterial epoxide hydrolase-catalyzed resolution of a 2,2-disubstituted oxirane:: optimization and upscaling

被引：28

作者：

Hellström, H ^{[1
]}

Steinreiber, A ^{[1
]}

Mayer, SF ^{[1
]}

Faber, K ^{[1
]}

机构：

[1] Graz Univ, Dept Chem Organ & Bioorgan Chem, A-8010 Graz, Austria

来源：

BIOTECHNOLOGY LETTERS | 2001年 / 23卷 / 03期

基金：

奥地利科学基金会;

关键词：

bacterial epoxide hydrolase; pH-optimization; Rhodococcus ruber; temperature-optimization;

D O I：

10.1023/A:1005636121060

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The epoxide hydrolase-catalyzed resolution of (+/-)-2-methylglycidyl benzyl ether, a versatile chiral building block for the asymmetric synthesis of bioactive compounds, mediated by whole cells of Rhodococcus ruber SM 1789 was accomplished. Among various parameters (such as temperature, buffer type, pH and catalyst/substrate-ratio) an elevated substrate-concentration proved to be particularly sensitive with respect to a significant enhancement of the enantioselectivity.

引用

页码：169 / 173

页数：5

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