Characterization of biomarkers in polycystic ovary syndrome (PCOS) using multiple distinct proteomic platforms

被引：32

作者：

Matharoo-Ball, B.

Hughes, C.

Lancashire, L.

Tooth, D.

Ball, G.

Creaser, C.

Elgasim, M.

Rees, R.

Layfield, R. ^{[1
]}

Atiomo, W.

机构：

[1] Univ Nottingham, Sch Med, Queens Med Ctr, Sch Biomed Sci, Nottingham NG7 2UH, England

[2] Nottingham Trent Univ, Sch Biomed & Nat Sci, Nottingham NG11 8NS, England

[3] Univ Nottingham, Sch Human Dev, Dept Obstet & Gynaecol, Nottingham NG7 2UH, England

[4] Novozymes Delta Ltd, Nottingham NG7 1FD, England

来源：

JOURNAL OF PROTEOME RESEARCH | 2007年 / 6卷 / 08期

关键词：

polycystic ovary syndrome; 2DE; MALDI-TOF MS; complement proteins; haptoglobin;

D O I：

10.1021/pr070124b

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A variety of prefractionation methods (including a novel reversed-phase solid-phase-extraction (RP-SPE) combined with SDS-PAGE and proteomic based approaches (e.g., 2-dimensional gel electrophoresis (2DE) and MALDI-TOF mass spectrometry combined with Artificial Neural Network (ANN) bioinformatic tools) were used to investigate the protein/pepticle signatures in patients with Polycystic Ovary Syndrome (PCOS). Four potential PCOS biomarkers were identified (complement C4 alpha 3c and C4 gamma and haptoglobin alpha and beta chains).

引用

页码：3321 / 3328

页数：8

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