A newly developed bisphosphonate, YM529, is a potent apoptosis inducer of human myeloma cells

被引：56

作者：

Takahashi, R ^{[1
]}

Shimazaki, C ^{[1
]}

Inaba, T ^{[1
]}

Okano, A ^{[1
]}

Hatsuse, M ^{[1
]}

Okamoto, A ^{[1
]}

Hirai, H ^{[1
]}

Ashihara, E ^{[1
]}

Nakagawa, M ^{[1
]}

机构：

[1] Kyoto Prefectural Univ Med, Dept Med 2, Kamigyo Ku, Kyoto 6028566, Japan

来源：

LEUKEMIA RESEARCH | 2001年 / 25卷 / 01期

基金：

日本学术振兴会;

关键词：

bisphosphonate; YM529; myeloma; apoptosis;

D O I：

10.1016/S0145-2126(00)00087-4

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We examined the effect of YM529, a newly developed third-generation bisphosphonate (BP), on the growth of human myeloma cell lines using the trypan blue dye exclusion test and Alamar blue assay. BPs induced inhibition of proliferation in all cell lines dose-dependently, and YM529 had a most potent growth inhibitory effect, followed by incadronate and pamidronate. Flow cytometric analysis using annexinV and 7AAD showed that YM529 most significantly induced apoptosis of all myeloma cell lines. These observations suggested that YM529 is a potent apoptosis inducer of myeloma cells, and might have some benefit not only on the improvement of bone lesions but also on survival in some myeloma patients. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：77 / 83

页数：7

共 32 条

[21]

REID IR, 1988, LANCET, V1, P143

[22]

Sasaki A, 1998, INT J CANCER, V77, P279, DOI 10.1002/(SICI)1097-0215(19980717)77:2<279::AID-IJC18>3.0.CO

[23]

2-6

[24] BISPHOSPHONATE ACTION - ALENDRONATE LOCALIZATION IN RAT BONE AND EFFECTS ON OSTEOCLAST ULTRASTRUCTURE [J].

SATO, M ;

GRASSER, W ;

ENDO, N ;

AKINS, R ;

SIMMONS, H ;

THOMPSON, DD ;

GOLUB, E ;

RODAN, GA .

JOURNAL OF CLINICAL INVESTIGATION, 1991, 88 (06) :2095-2105

[25]

Shipman CM, 1998, CANCER RES, V58, P5294

[26] Bisphosphonates induce apoptosis in human myeloma cell lines: a novel anti-tumour activity [J].

Shipman, CM ;

Rogers, MJ ;

Apperley, JF ;

Graham, R ;

Russell, G ;

Croucher, PI .

BRITISH JOURNAL OF HAEMATOLOGY, 1997, 98 (03) :665-672

[27]

SMITH R, 1971, LANCET, V1, P945

[28]

Takeuchi M, 1998, CHEM PHARM BULL, V46, P1703

[29] SENSITIVE DETERMINATION OF A NOVEL BISPHOSPHONATE, YM529, IN PLASMA, URINE AND BONE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION [J].

USUI, T ;

KAWAKAMI, R ;

WATANABE, T ;

HIGUCHI, S .

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS, 1994, 652 (01) :67-72

[30] Bisphosphonates inhibit the adhesion of breast cancer cells to bone matrices in vitro [J].

vanderPluijm, G ;

Vloedgraven, H ;

vanBeek, E ;

vanderWeePals, L ;

Lowik, C ;

Papapoulos, S .

JOURNAL OF CLINICAL INVESTIGATION, 1996, 98 (03) :698-705

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