Vitamin E inhibits proliferation of human Tenon's capsule fibroblasts in vitro

被引：34

作者：

Haas, AL ^{[1
]}

Boscoboinik, D ^{[1
]}

Mojon, DS ^{[1
]}

Bohnke, M ^{[1
]}

Azzi, A ^{[1
]}

机构：

[1] UNIV BERN,INST BIOCHEM & MOL BIOL,BERN,SWITZERLAND

来源：

OPHTHALMIC RESEARCH | 1996年 / 28卷 / 03期

关键词：

trabeculectomy; Tenon's capsule fibroblasts; cell culture; vitamin E; d-alpha-tocopherol; antioxidants;

D O I：

10.1159/000267899

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

Failure of glaucoma surgery is mostly due to fibrocellular scar formation, derived from Tenon's capsule fibroblasts. In highrisk cases, postoperative Tenon's capsule fibroblast proliferation is inhibited by mitomycin C or 5-fluorouracil. Toxicity to other ocular cell types and the risk of ocular hypotony limits the use of these agents. We have found that d-alpha-tocopherol (vitamin E) was able to inhibit proliferation of in vitro human Tenon's capsule fibroblasts obtained from seven different donors. At 48 h, inhibition of cell proliferation was 30-78% (mean 60%) for 50 mu M d-alpha-tocopherol and 46-97% (mean 77%) for 100 mu M d-alpha-tocopherol. This inhibition was statistically significant. No cytotoxic effects were observed.

引用

页码：171 / 175

页数：5

共 15 条

[1] D-ALPHA-TOCOPHEROL CONTROL OF CELL-PROLIFERATION [J].

AZZI, A ;

BOSCOBOINIK, D ;

CHATELAIN, E ;

OZER, NK ;

STAUBLE, B .

MOLECULAR ASPECTS OF MEDICINE, 1993, 14 (03) :265-271

[2]

BENDICH A, 1992, ANN NY ACAD SCI, V669, P300

[3] THE UPTAKE OF (R,R,R)ALPHA-TOCOPHEROL BY HUMAN-ENDOTHELIAL CELLS IN CULTURE [J].

CHAN, AC ;

TRAN, K .

LIPIDS, 1990, 25 (01) :17-21

[4] INHIBITION OF SMOOTH-MUSCLE CELL-PROLIFERATION AND PROTEIN-KINASE-C ACTIVITY BY TOCOPHEROLS AND TOCOTRIENOLS [J].

CHATELAIN, E ;

BOSCOBOINIK, DO ;

BARTOLI, GM ;

KAGAN, VE ;

GEY, FK ;

PACKER, L ;

AZZI, A .

BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1176 (1-2) :83-89

[5]

Filina A A, 1994, Vestn Oftalmol, V110, P33

[6]

Glantz S.A., 1981, PRIMER BIOSTATISTICS

[7]

JAMPEL HD, 1988, ARCH OPHTHALMOL-CHIC, V106, P89

[8]

LEE DA, 1990, INVEST OPHTH VIS SCI, V31, P2136

[9]

MALLICK KS, 1985, ARCH OPHTHALMOL-CHIC, V103, P1398

[10]

Mietz H, 1994, Ger J Ophthalmol, V3, P164

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