Direct indication for the existence of a double stalk in CF0F1

被引：83

作者：

Böttcher, B ^{[1
]}

Schwarz, L ^{[1
]}

Gräber, P ^{[1
]}

机构：

[1] Univ Freiburg, Inst Phys Chem, D-79104 Freiburg, Germany

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1998年 / 281卷 / 05期

关键词：

H+-ATPase; stalk; rotor-stator-model; electron microscopy; image processing;

D O I：

10.1006/jmbi.1998.1957

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The H+-ATPase from chloroplasts (CF0F1) was investigated by electron microscopy of negatively stained single molecules followed by image processing. The analysis of about 4700 particles from 72 micrographs gave clear evidence that the membrane-integrated F-0 part is connected by at least two stalks to the F-1 part. One of the two stalks is more prominent and connects a central part of F-1 with a slightly peripheral part of F-0. The other stalk connects a peripheral part of F-1 to a peripheral part of F-1. (C) Academic Press.

引用

页码：757 / 762

页数：6

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