Strengths and limitations of commercial tests for hepatitis C virus RNA quantification

被引：27

作者：

Morishima, C

Chung, M

Ng, KW

Brambilla, DJ

Gretch, DR

机构：

[1] Univ Washington, Harborview Med Ctr, Dept Pediat, Sch Med, Seattle, WA 98104 USA

[2] Univ Washington, Dept Lab Med, Sch Med, Seattle, WA 98104 USA

[3] Univ Washington, Dept Med, Sch Med, Seattle, WA 98104 USA

[4] New England Res Inst, Watertown, MA 02172 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2004年 / 42卷 / 01期

关键词：

D O I：

10.1128/JCM.42.1.421-425.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The sensitivity of the Roche COBAS Monitor v.2.0 test was slightly better than that of the Bayer bDNA 3.0 test, but the Monitor test underquantified specimens by 2.5- to 10.6-fold even at relatively low hepatitis C virus RNA concentrations. Dilution prior to assay minimally decreased the reproducibility of the Monitor assay, leading to the recommendation for all specimens to be diluted 1:100 prior to testing by this assay.

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收藏

页码：421 / 425

页数：5

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