Prospective study of human metapneumovirus detection in clinical samples by use of light diagnostics direct immunofluorescence reagent and real-time PCR

被引：21

作者：

Landry, Marie L.

Cohen, Sandra

Ferguson, David

机构：

[1] Yale Univ, Sch Med, Dept Lab Med, New Haven, CT 06520 USA

[2] Yale New Haven Med Ctr, Dept Lab Med, New Haven, CT 06504 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2008年 / 46卷 / 03期

关键词：

D O I：

10.1128/JCM.01926-07

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A Light Diagnostics human metapneumovirus (HMPV) monoclonal antibody reagent was evaluated using cytospin-enhanced direct immunofluorescence (DFA), and the results were compared to those for TaqMan reverse transcription-PCR (RT-PCR). Of the 202 samples tested, 48 were positive by RT-PCR, and 41 (85.4%) of these were positive by DFA (P = 0.0771). The commercial availability of an HMPV DFA reagent will be of significant benefit to clinical laboratories.

引用

页码：1098 / 1100

页数：3

共 16 条

[11] SimulFluor respiratory screen for rapid detection of multiple respiratory viruses in clinical specimens by immunofluorescence staining [J].