Strategies for direct attachment of hapten to a polystyrene support for applications in enzyme-linked immunosorbent assay (ELISA)

被引：35

作者：

Kaur, J ^{[1
]}

Singh, KV ^{[1
]}

Raje, M ^{[1
]}

Varshney, GC ^{[1
]}

Suri, CR ^{[1
]}

机构：

[1] Inst Microbial Technol, Chandigarh 160036, India

来源：

ANALYTICA CHIMICA ACTA | 2004年 / 506卷 / 02期

关键词：

hapten; polystyrene; silanization; ELISA;

D O I：

10.1016/j.aca.2003.11.009

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Anew method describing direct attachment of carboxylated haptens on a polystyrene support, using 3-aminopropyltriethoxysilane (3-APTES) as a linker, is reported. The hapten coated polystyrene support showed excellent stability as a function of the buffer pH and reaction time, and was successfully used to demonstrate its application in enzyme-linked immunosorbent assay (ELISA). (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：133 / 135

页数：3

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