Isolation and characterization of As60A, a transforming growth factor-β gene, from the malaria vector Anopheles stephensi

被引:8
作者
Crampton, AL [1 ]
Luckhart, S [1 ]
机构
[1] Virginia Tech, Dept Biochem, Blacksburg, VA 24061 USA
基金
美国国家卫生研究院;
关键词
TGF-beta/60A; mosquito; malaria; arthropod;
D O I
10.1006/cyto.2000.0801
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have isolated the first mosquito member of the TGF-beta superfamily, As60A, As60A is a single copy gene, similar to 5kb in length and encodes eight exons. Here we report the isolation and characterization of two of four transcripts produced from this gene. The transcripts As60A(1) and As60A(2) encode related 5'UTR/exon 1 sequences. As60A is most similar to the 60A genes from Drosophila and is thus a member of the Dpp/BMP subfamily of the TGF-beta superfamily, The splice junction of intron 2 is conserved among As60A, BMP2, BMP4, Tc-Dpp, Bm-tgh-1, TGF-beta1 and Dpp, Intron 2 also contains three putative binding sites for a DorsaUGambif1 transcription factor. The large number of introns and the conservation of intron 2 indicate that As60A is relatively ancient compared to the other arthropod TGF-beta genes. We also propose that As60A plays a role in the mosquito immune response to Plasmodium infection. (C) 2001 Academic Press.
引用
收藏
页码:65 / 74
页数:10
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