A Chemiluminescent Antibody Array System for Detection of Foodborne Pathogens in Milk

被引：19

作者：

Charlermroj, Ratthaphol ^{[1
]}

Gajanandana, Oraprapai ^{[1
]}

Barnett, Charlie ^{[2
]}

Kirtikara, Kanyawim ^{[1
]}

Karoonuthaisiri, Nitsara ^{[1
]}

机构：

[1] NSTDA, Natl Ctr Genet Engn & Biotechnol BIOTEC, Pathum Thani 12120, Thailand

[2] Nano Detect Technol Inc, Knoxville, TN USA

来源：

ANALYTICAL LETTERS | 2011年 / 44卷 / 06期

关键词：

Antibody array; Chemiluminescence; E. coli O157:H7; Immunoassay; Salmonella spp. milk; ESCHERICHIA-COLI O157-H7; LEGIONELLA-PNEUMOPHILA; LISTERIA-MONOCYTOGENES; MICROARRAY; SALMONELLA;

D O I：

10.1080/00032719.2010.511736

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学];

摘要：

A foodborne antibody array (FAbA) was developed as a rapid, inexpensive, and accurate detection to simultaneously detect Escherichia coli O157:H7 and Salmonella spp. using a custom-made chemiluminescent detector. We have optimized fabrication processes, selected the antibody concentrations, calibrated the signals to the pathogen amount, and minimized assay time. In comparison with 4 hours for ELISA, FAbA takes only 75 minutes to achieve relatively the same sensitivities (8x104 and 5x107CFU/ml for E. coli and Salmonella, respectively). The FAbA was able to detect the pathogens in milk at the same detection limits as in the buffer.

引用

页码：1085 / 1099

页数：15

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