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Methylation of TIMP3 in esophageal squamous cell carcinoma

被引：14
作者：
Smith, Eric [1 ]
De Young, Neville J.
Tian, Zi-Qiang [2 ]
Caruso, Maria [3 ]
Ruszkiewicz, Andrew R. [3 ]
Liu, Jun-Feng [2 ]
Jamieson, Glyn G.
Drew, Paul A. [4 ]
机构：
[1] Univ Adelaide, Sch Med, Fac Hlth Sci, Discipline Surg,Royal Adelaide Hosp, Adelaide, SA 5005, Australia
[2] Hebei Med Univ, Hosp 4, Dept Thorac Surg, Shijiazhuang, Hebei Province, Peoples R China
[3] Inst Med & Vet Sci, Div Tissue Pathol, Adelaide, SA 5000, Australia
[4] Flinders Univ S Australia, Sch Nursing & Midwifery, Bedford Pk, SA 5042, Australia
来源：
WORLD JOURNAL OF GASTROENTEROLOGY | 2008年 / 14卷 / 02期
关键词：
esophageal squamous cell carcinoma; immunohistochemistry; methylation; TIMP3;
D O I：
10.3748/wjg.14.203
中图分类号：
R57 [消化系及腹部疾病];
学科分类号：
摘要：
AIM: To measure the frequency of DNA methylation of the tissue inhibitor of metalloproteinase 3 (TIMP3) promoter and relate this to any change of gene expression in esophageal squamous cell carcinoma in patients from a region of high incidence in China. METHODS: Cancer cell lines were treated with or without the demethylating reagent 5-aza-2'-deoxycytidine. Methylation of the TIMP3 promoter was assessed in three regions by melt curve analysis and its expression was assessed by real-time RT-PCR. Tumors and proximal resection margins were obtained from 64 patients with esophageal squamous cell carcinoma from a region of high incidence in China. Methylation was assessed by melt curve analysis and expression by immunohistochemistry. RESULTS: Methylation in one of the three promoter regions assessed correlated with gene silencing in esophageal cell lines. A degree of methylation of TIMP3 was found in only four esophageal squamous cell carcinomas, and partial loss of TIMP3 protein expression in just one. CONCLUSION: Methylation and loss of expression of TIMP3 occurs infrequently in esophageal squamous cell carcinoma in a region of high incidence in China. (c) 2008 WJG. All rights reserved.
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页码：203 / 210
页数：8
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