Methylation of TIMP3 in esophageal squamous cell carcinoma

被引：14

作者：

Smith, Eric ^{[1
]}

De Young, Neville J.

Tian, Zi-Qiang ^{[2
]}

Caruso, Maria ^{[3
]}

Ruszkiewicz, Andrew R. ^{[3
]}

Liu, Jun-Feng ^{[2
]}

Jamieson, Glyn G.

Drew, Paul A. ^{[4
]}

机构：

[1] Univ Adelaide, Sch Med, Fac Hlth Sci, Discipline Surg,Royal Adelaide Hosp, Adelaide, SA 5005, Australia

[2] Hebei Med Univ, Hosp 4, Dept Thorac Surg, Shijiazhuang, Hebei Province, Peoples R China

[3] Inst Med & Vet Sci, Div Tissue Pathol, Adelaide, SA 5000, Australia

[4] Flinders Univ S Australia, Sch Nursing & Midwifery, Bedford Pk, SA 5042, Australia

来源：

WORLD JOURNAL OF GASTROENTEROLOGY | 2008年 / 14卷 / 02期

关键词：

esophageal squamous cell carcinoma; immunohistochemistry; methylation; TIMP3;

D O I：

10.3748/wjg.14.203

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

AIM: To measure the frequency of DNA methylation of the tissue inhibitor of metalloproteinase 3 (TIMP3) promoter and relate this to any change of gene expression in esophageal squamous cell carcinoma in patients from a region of high incidence in China. METHODS: Cancer cell lines were treated with or without the demethylating reagent 5-aza-2'-deoxycytidine. Methylation of the TIMP3 promoter was assessed in three regions by melt curve analysis and its expression was assessed by real-time RT-PCR. Tumors and proximal resection margins were obtained from 64 patients with esophageal squamous cell carcinoma from a region of high incidence in China. Methylation was assessed by melt curve analysis and expression by immunohistochemistry. RESULTS: Methylation in one of the three promoter regions assessed correlated with gene silencing in esophageal cell lines. A degree of methylation of TIMP3 was found in only four esophageal squamous cell carcinomas, and partial loss of TIMP3 protein expression in just one. CONCLUSION: Methylation and loss of expression of TIMP3 occurs infrequently in esophageal squamous cell carcinoma in a region of high incidence in China. (c) 2008 WJG. All rights reserved.

引用

页码：203 / 210

页数：8

共 38 条

[11] Matrix metalloproteinases and tumor metastasis [J].