Adjuvanticity of compound polysaccharides on chickens against Newcastle disease and avian influenza vaccine

被引：68

作者：

Guo, Liwei ^{[1
]}

Wang, Deyun ^{[1
]}

Hu, Yuanliang ^{[1
]}

Zhao, Xiaona ^{[1
,3
]}

Wang, Yuanlei ^{[1
]}

Yang, Shujuan ^{[1
]}

Wang, Junmin ^{[1
]}

Fan, Yunpeng ^{[1
]}

Han, Guocai ^{[2
]}

Gao, Huan ^{[1
]}

机构：

[1] Nanjing Agr Univ, Inst Tradit Chinese Vet Med, Coll Vet Med, Nanjing 210095, Jiangsu, Peoples R China

[2] China Agr Univ, Coll Anim Sci & Technol, Beijing 100193, Peoples R China

[3] Shandong Agr Univ, Coll Anim Sci & Vet Med, Tai An 271018, Shandong, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2012年 / 50卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Astragalus polysaccharides; Sulfated Epimedium polysaccharides; Newcastle disease vaccine; Avian influenza vaccine; Immunity; Chicken; HERBAL MEDICINAL INGREDIENTS; SULFATED MODIFICATION; ENHANCE;

D O I：

10.1016/j.ijbiomac.2012.01.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

This study was conducted to evaluate the effects of compound polysaccharides (cPS) on the immune responses via chicken models. First, in screening experiment, a comprehensive analysis for immunomodulatory activity of four cPSs, including Astragalus polysaccharides CAPS), Epimedium polysaccharides (EPS), sulfated APS (sAPS) and sulfated EPS (sEPS), was performed in vitro and in vivo. APS-sEPS was picked out having the best effect on lymphocyte proliferation and raising the antibody titers. Therefore, the adjuvanticities of APS-sEPS on Newcastle disease (ND) and avian influenza (AI) vaccine were further validated. Chickens were administrated with ND or AI vaccines containing APS-sEPS of 150, 100 and 50 mg/kg, respectively, taking oil adjuvant vaccine as control. It was observed ND or AI antibody titers and lymphocyte proliferation were enhanced at 100 mg/kg of APS-sEPS. In conclusion, appropriate dose of APS-sEPS may be a safe and efficacious immune stimulator candidate suitable for vaccines. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：512 / 517

页数：6

共 38 条

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