Glutamic acid independent production of poly(gamma-glutamic acid) by Bacillus subtilis TAM-4

被引：75

作者：

Ito, Y ^{[1
]}

Tanaka, T ^{[1
]}

Ohmachi, T ^{[1
]}

Asada, Y ^{[1
]}

机构：

[1] HIROSAKI UNIV, FAC AGR, DEPT BIORESOURCE SCI, HIROSAKI, AOMORI 036, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1996年 / 60卷 / 08期

关键词：

Bacillus subtilis; poly(gamma-glutamic acid);

D O I：

10.1271/bbb.60.1239

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A bacterium that produced a large amount of poly(gamma-glutamic acid) (PGA) when it was grown aerobically in a culture medium containing ammonium salt and sugar as sources of nitrogen and carbon, respectively, was isolated from soil. The bacterium, strain TAM-4, was classified as Bacillus subtilis. The maximum PGA production (22.1 mg/ml) was obtained when it was grown in a medium containing 1.8% ammonium chloride and 7.5% fructose at 30 degrees C for 96 h with shaking. Some properties of the PGA obtained at different times of cultivation were investigated by gel permeation chromatography, SDS-PAGE, and measurement of viscosity, and calculation of the D/L ratio of glutamic acid constituting PGA. The results suggested that PGA was elongated with no changes in the diastereoisomer ratio in the molecule.

引用

页码：1239 / 1242

页数：4

共 26 条

[1] TRANSFORMATION OF BACILLUS-SUBTILIS IN POLYGLUTAMATE PRODUCTION BY DEOXYRIBONUCLEIC-ACID FROM BACILLUS-NATTO [J].

AUMAYR, A ;

HARA, T ;

UEDA, S .

JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, 1981, 27 (02) :115-123

[2] GAMMA-POLY(GLUTAMIC ACID) FORMATION BY BACILLUS-LICHENIFORMIS 9945A - PHYSIOLOGICAL AND BIOCHEMICAL-STUDIES [J].

BIRRER, GA ;

CROMWICK, AM ;

GROSS, RA .

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 1994, 16 (05) :265-275

[3]

Bovarnick M, 1942, J BIOL CHEM, V145, P415

[4] PRODUCTION OF GAMMA-POLYGLUTAMIC ACID BY BACILLUS-LICHENIFORMIS A35 UNDER DENITRIFYING CONDITIONS [J].

CHENG, C ;

ASADA, Y ;

AIDA, T .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1989, 53 (09) :2369-2375

[5] COLORIMETRIC METHOD FOR DETERMINATION OF SUGARS AND RELATED SUBSTANCES [J].

DUBOIS, M ;

GILLES, KA ;

HAMILTON, JK ;

REBERS, PA ;

SMITH, F .

ANALYTICAL CHEMISTRY, 1956, 28 (03) :350-356

[6] BIOSYNTHESIS AND HYDROLYSIS OF POLY(GAMMA-GLUTAMIC ACID) FROM BACILLUS-SUBTILIS IFO3335 [J].

GOTO, A ;

KUNIOKA, M .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1992, 56 (07) :1031-1035

[7]

*JAP STAND ASS, 1993, 01021993 JIS K, P147

[8] PRODUCTION OF POLY(GAMMA-GLUTAMIC ACID) BY BACILLUS-SUBTILIS F-2-01 [J].

KUBOTA, H ;

MATSUNOBU, T ;

UOTANI, K ;

TAKEBE, H ;

SATOH, A ;

TANAKA, T ;

TANIGUCHI, M .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1993, 57 (07) :1212-1213

[9]

KUNIOKA M, 1994, APPL MICROBIOL BIOT, V40, P867, DOI 10.1007/s002530050082

[10] PROPERTIES OF HYDROGELS PREPARED BY GAMMA-IRRADIATION IN MICROBIAL POLY(GAMMA-GLUTAMIC ACID) AQUEOUS-SOLUTIONS [J].

KUNIOKA, M .

KOBUNSHI RONBUNSHU, 1993, 50 (10) :755-760

← 1 2 3 →