Arbuscular mycorrhizal symbiosis increases relative apoplastic water flow in roots of the host plant under both well-watered and drought stress conditions

被引:158
作者
Barzana, Gloria [1 ]
Aroca, Ricardo [1 ]
Antonio Paz, Jose [1 ]
Chaumont, Francois [2 ]
Carmen Martinez-Ballesta, Mari [3 ]
Carvajal, Micaela [3 ]
Manuel Ruiz-Lozano, Juan [1 ]
机构
[1] Estn Expt Zaidin EEZ CSIC, Dept Microbiol Suelo & Sistemas Simbiot, Granada 18008, Spain
[2] Catholic Univ Louvain, Inst Sci Vie, B-1348 Louvain, Belgium
[3] Ctr Edafol & Biol Aplicada Segura CEBAS CSIC, Dept Plant Nutr, Murcia 30100, Spain
关键词
Apoplastic pathway; arbuscular mycorrhiza; cell-to-cell pathway; drought; root hydraulic conductivity; LACTUCA-SATIVA PLANTS; HYDRAULIC-PROPERTIES; EXOGENOUS ABA; GAS-EXCHANGE; REFLECTION COEFFICIENT; GENE-EXPRESSION; LEAF GROWTH; TRANSPORT; AQUAPORINS; CONDUCTANCE;
D O I
10.1093/aob/mcs007
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Background and Aims The movement of water through mycorrhizal fungal tissues and between the fungus and roots is little understood. It has been demonstrated that arbuscular mycorrhizal (AM) symbiosis regulates root hydraulic properties, including root hydraulic conductivity. However, it is not clear whether this effect is due to a regulation of root aquaporins (cell-to-cell pathway) or to enhanced apoplastic water flow. Here we measured the relative contributions of the apoplastic versus the cell-to-cell pathway for water movement in roots of AM and non-AM plants. Methods We used a combination of two experiments using the apoplastic tracer dye light green SF yellowish and sodium azide as an inhibitor of aquaporin activity. Plant water and physiological status, root hydraulic conductivity and apoplastic water flow were measured. Key Results Roots of AM plants enhanced significantly relative apoplastic water flow as compared with non-AM plants and this increase was evident under both well-watered and drought stress conditions. The presence of the AM fungus in the roots of the host plants was able to modulate the switching between apoplastic and cell-to-cell water transport pathways. Conclusions The ability of AM plants to switch between water transport pathways could allow a higher flexibility in the response of these plants to water shortage according to the demand from the shoot.
引用
收藏
页码:1009 / 1017
页数:9
相关论文
共 54 条
[11]   Mannitol permeation and radial flow of water in maize roots [J].
Fritz, Michael ;
Ehwald, Rudolf .
NEW PHYTOLOGIST, 2011, 189 (01) :210-217
[12]  
Giovannetti M., 1980, NEW PHYTOL, V84, P489, DOI DOI 10.1111/J.1469-8137.1980.TB04556.X
[13]  
Goicoechea N, 1997, PHYSIOL PLANTARUM, V100, P989, DOI [10.1034/j.1399-3054.1997.1000427.x, 10.1111/j.1399-3054.1997.tb00027.x]
[14]   Localization and quantification of plasma membrane aquaporin expression in maize primary root: A clue to understanding their role as cellular plumbers [J].
Hachez, Charles ;
Moshelion, Menachem ;
Zelazny, Enric ;
Cavez, Damien ;
Chaumont, Francois .
PLANT MOLECULAR BIOLOGY, 2006, 62 (1-2) :305-323
[15]  
Hoagland D. R., 1938, Annual Report of the Smithsonian Institution, P461
[16]   Metabolic inhibition of root water flow in red-osier dogwood (Cornus stolonifera) seedlings [J].
Kamaluddin, M ;
Zwiazek, JJ .
JOURNAL OF EXPERIMENTAL BOTANY, 2001, 52 (357) :739-745
[17]   Quantification of water uptake by arbuscular mycorrhizal hyphae and its significance for leaf growth, water relations, and gas exchange of barley subjected to drought stress [J].
Khalvati, MA ;
Hu, Y ;
Mozafar, A ;
Schmidhalter, U .
PLANT BIOLOGY, 2005, 7 (06) :706-712
[18]   Water uptake by seminal and adventitious roots in relation to whole-plant water flow in barley (Hordeum vulgare L.) [J].
Knipfer, Thorsten ;
Fricke, Wieland .
JOURNAL OF EXPERIMENTAL BOTANY, 2011, 62 (02) :717-733
[19]   Root pressure and a solute reflection coefficient close to unity exclude a purely apoplastic pathway of radial water transport in barley (Hordeum vulgare) [J].
Knipfer, Thorsten ;
Fricke, Wieland .
NEW PHYTOLOGIST, 2010, 187 (01) :159-170
[20]   Role of aquaporins in root water transport of ectomycorrhizal jack pine (Pinus banksiana) seedlings exposed to NaCl and fluoride [J].
Lee, Seong Hee ;
Calvo-Polanco, Monica ;
Chung, Gap Chae ;
Zwiazek, Janusz J. .
PLANT CELL AND ENVIRONMENT, 2010, 33 (05) :769-780