Automated on-line SPE LC-MS/MS method to quantitate 6beta-hydroxycortisol and cortisol in human urine: Use of the 6beta-hydroxycortisol to cortisol ratio as an indicator of CYP3A4 activity

被引：33

作者：

Barrett, YC ^{[1
]}

Akinsanya, B ^{[1
]}

Chang, SY ^{[1
]}

Vesterqvist, O ^{[1
]}

机构：

[1] Bristol Myers Squibb Co, Clin Discovery, Princeton, NJ 08543 USA

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2005年 / 821卷 / 02期

关键词：

6beta-hydroxycortisol; cortisol; LC-MS/MS; CYP3A4; human urine;

D O I：

10.1016/j.jchromb.2005.04.030

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive method for quantitation of urinary 6beta-hydroxycortisol (6beta-HC) and cortisol using on-line SPE and LC-MS/MS was developed and validated. Human urine samples were injected directly onto an on-line solid phase extraction apparatus, Prospekt-2, followed by HPLC separation and electrospray triple quadrupole LC-MS/MS detection. The inter-day precision for the 6beta-HC:cortisol ratio was 7-9%. The lower limit of quantitation was 1 and 0.2 ng/mL for 6beta-HC and cortisol, respectively. Using the method we observed a diurnal variation on the 6beta-HC:cortisol ratio in healthy volunteers with the maximal ratio observed in the 2-10 pm urine collection period. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：159 / 165

页数：7

共 21 条

[1] SERUM CREATININE DETERMINATION WITHOUT PROTEIN PRECIPITATION [J].

BARTELS, H ;

BOHMER, M ;

HEIERLI, C .

CLINICA CHIMICA ACTA, 1972, 37 (NMAR) :193-&

[2]

BURTIS C, 2001, TIETS FUNDAMENTALS C, P40

[3] ROUTINE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF URINARY UNCONJUGATED CORTISOL USING SOLID-PHASE EXTRACTION AND ULTRAVIOLET DETECTION [J].

DOLEZALOVA, M .

CLINICA CHIMICA ACTA, 1994, 231 (02) :129-137

[4]

Eeckhoudt SL, 2001, INT J CLIN PHARM TH, V39, P293

[5] Evidence for the validity of cortisol 6β-hydroxylation clearance as a new index for in vivo cytochrome P450 3A phenotyping in humans [J].

Furuta, T ;

Suzuki, A ;

Mori, C ;

Shibasaki, H ;

Yokokawa, A ;

Kasuya, Y .

DRUG METABOLISM AND DISPOSITION, 2003, 31 (11) :1283-1287

[6] Urinary 6β-hydroxycortisol:: a validated test for evaluating drug induction or drug inhibition mediated through CYP3A in humans and in animals [J].

Galteau, MM ;

Shamsa, F .

EUROPEAN JOURNAL OF CLINICAL PHARMACOLOGY, 2003, 59 (10) :713-733

[7] THE INCREASE IN URINARY-EXCRETION OF 6-BETA-HYDROXYCORTISOL AS A MARKER OF HUMAN HEPATIC CYTOCHROME-P450IIIA INDUCTION [J].

GED, C ;

ROUILLON, JM ;

PICHARD, L ;

COMBALBERT, J ;

BRESSOT, N ;

BORIES, P ;

MICHEL, H ;

BEAUNE, P ;

MAUREL, P .

BRITISH JOURNAL OF CLINICAL PHARMACOLOGY, 1989, 28 (04) :373-387

[8] SIMULTANEOUS HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF 6-BETA-HYDROXYCORTISOL AND CORTISOL IN URINE WITH FLUORESCENCE DETECTION AND ITS APPLICATION FOR ESTIMATING HEPATIC DRUG-METABOLIZING ENZYME-INDUCTION [J].

INOUE, S ;

INOKUMA, M ;

HARADA, T ;

SHIBUTANI, Y ;

YOSHITAKE, T ;

CHARLES, B ;

ISHIDA, J ;

YAMAGUCHI, M .

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS, 1994, 661 (01) :15-23

[9]

Lee C, 1996, CLIN CHEM, V42, P1290

[10] SIMULTANEOUS DETERMINATION OF URINARY FREE CORTISOL AND 6-BETA-HYDROXYCORTISOL BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY TO MEASURE HUMAN CYP3A ACTIVITY [J].

LYKKESFELDT, J ;

LOFT, S ;

POULSEN, HE .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 1994, 660 (01) :23-29

← 1 2 3 →