Optimization of diphasic dialysis procedure for clenbuterol residues extraction in bovine retina and hair

Development of a simple, rapid, and accurate methodology for clenbuterol residue extraction in bovine hair and retina by diphasic dialysis was evaluated. After bovine hair and retina samples digestion, diphasic dyalisis was used as an extraction procedure using four organic solvents (dichloromethane, n-hexane, ethyl acetate, and diethyl ether) and five different buffers (acetate, phosphate, berate, carbonate, and citrate). The organic extract was evaporated to dryness under a nitrogen stream and the residue was derivatized with butylboronic acid. The results of GCMS determination show that acetate buffer [sodium acetate 0.2M: acetic acid (95:5) pH approximate to 5.8] was the best choice for buffer dialysis, when dichloromethane and diethyl ether were elected as extraction organic diphasic dialysis solvents for hair and retina, respectively. Discussion of validation data show that diphasic dialysis could be a good, rapid, accurate, and economic extraction procedure to determine clenbuterol in bovine hair and retina samples.