FISHing with locked nucleic acids (LNA): evaluation of different LNA/DNA mixmers

被引：45

作者：

Silahtaroglu, AN

Tommerup, N

Vissing, H

机构：

[1] Univ Copenhagen, Panum Inst,IMBG, Dept Med Genet, Wilhelm Johannsen Ctr Funct Genome Res, DK-2200 Copenhagen, Denmark

[2] Exiqon AS, DK-2950 Vedboek, Denmark

来源：

MOLECULAR AND CELLULAR PROBES | 2003年 / 17卷 / 04期

关键词：

locked nucleic acids; LNA; FISH; satellite-2;

D O I：

10.1016/S0890-8508(03)00048-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Locked Nucleic Acids (LNA) constitute a novel class of DNA analogues that have an exceptionally high affinity towards complementary DNA and RNA. Using human classical satellite-2 repeat sequence clusters as targets, we demonstrate that LNA/DNA mixmers oligonucleotides are excellent probes for FISH combining high binding affinity with short hybridization time and even with the ability to hybridize without prior thermal denaturation of the template. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：165 / 169

页数：5

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