Enzyme-assisted preparation of isotope-labeled 1-deoxy-D-xylulose 5-phosphate

被引：32

作者：

Hecht, S ^{[1
]}

Kis, F ^{[1
]}

Eisenreich, W ^{[1
]}

Amslinger, S ^{[1
]}

Wungsintaweekul, J ^{[1
]}

Herz, S ^{[1
]}

Rohdich, F ^{[1
]}

Bacher, A ^{[1
]}

机构：

[1] Tech Univ Munich, Lehrstuhl Organ Chem & Biochem, D-85747 Garching, Germany

来源：

JOURNAL OF ORGANIC CHEMISTRY | 2001年 / 66卷 / 11期

关键词：

D O I：

10.1021/jo0100300

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Recombinant l-deoxy-D-xylulose 5-phosphate synthase of Bacillus subtilis was used for the preparation of isotope-labeled 1-deoxy-D-xylulose 5-phosphate using isotope-labeled glucose and/ or isotope-labeled pyruvate as starting materials. The simple one-pot methods described afford almost every conceivable isotopomer of l-deoxy-rt-xylulose 5-phosphate carrying C-13 Or C-14 from commercially available precursors with an overall yield around 50%.

引用

页码：3948 / 3952

页数：5

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