Effect of metallothioneins on transformation of gelatinase A from human fibroblast WI-38 cells

被引：11

作者：

Haga, A

Nagase, H

Kito, H

Sato, T

机构：

[1] Department of Public Health, Gifu Pharmaceutical University, Gifu 502

来源：

CANCER LETTERS | 1996年 / 105卷 / 02期

关键词：

metallothionein; gelatinase A; WI-38; cells; tumor metastasis;

D O I：

10.1016/0304-3835(96)04277-2

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The effect of metallothioneins (MTs) on gelatinase A activity was investigated. The collagenolytic activity of gelatinase A from human fibroblast WI-38 cells was enhanced by the addition of MTs. This enhancement may be caused by the transformation of the inactive 62 kDa intermediate species of gelatinase A to the 59 kDa active enzyme. This enhancement was also observed in the conditioned medium of WI-38 cells exposed to heavy metals, but intracellular 72 kDa pro-gelatinase A did not change. Furthermore, degradation of gelatinase A occurred in the reaction between gelatinase A with substrate and MTs. Our results suggest that MTs may be an endogenous activator of gelatinase A, and may provide a host factor in cancer metastasis.

引用

页码：175 / 180

页数：6

共 33 条

[1] ASSOCIATION OF MMP-2 ACTIVATION POTENTIAL WITH METASTATIC PROGRESSION IN HUMAN BREAST-CANCER CELL-LINES INDEPENDENT OF MMP-2 PRODUCTION [J].