Proteomic analysis of cellular signaling

被引：28

作者：

Chen, WG ^{[1
]}

White, FM ^{[1
]}

机构：

[1] Inst Technol, Dept Biol Engn, Cambridge, MA 02139 USA

来源：

EXPERT REVIEW OF PROTEOMICS | 2004年 / 1卷 / 03期

关键词：

affinity chromatography; electrospray ionization; liquid chromatography; mass spectrometry; protein phosphorylation; proteomics; tandem mass spectrometry;

D O I：

10.1586/14789450.1.3.343

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Protein phosphorylation events are key regulators of cellular signaling processes. In the era of functional genomics, rational drug design programs demand large-scale high-throughput analysis of signal transduction cascades. Significant improvements in the area of mass spectrometry-based proteomics have provided exciting opportunities for rapid progress toward global protein phosphorylation analysis. This review summarizes several recent advances made in the field of phosphoproteomics with an emphasis placed on mass spectrometry instrumentation, enrichment methods and quantification strategies. In the near future, these technologies will provide a tool that can be used for quantitative investigation of signal transduction pathways to generate new insights into biologic systems.

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收藏

页码：343 / 354

页数：12

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