Proteomic analysis of cellular signaling

被引：28

作者：

Chen, WG ^{[1
]}

White, FM ^{[1
]}

机构：

[1] Inst Technol, Dept Biol Engn, Cambridge, MA 02139 USA

来源：

EXPERT REVIEW OF PROTEOMICS | 2004年 / 1卷 / 03期

关键词：

affinity chromatography; electrospray ionization; liquid chromatography; mass spectrometry; protein phosphorylation; proteomics; tandem mass spectrometry;

D O I：

10.1586/14789450.1.3.343

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Protein phosphorylation events are key regulators of cellular signaling processes. In the era of functional genomics, rational drug design programs demand large-scale high-throughput analysis of signal transduction cascades. Significant improvements in the area of mass spectrometry-based proteomics have provided exciting opportunities for rapid progress toward global protein phosphorylation analysis. This review summarizes several recent advances made in the field of phosphoproteomics with an emphasis placed on mass spectrometry instrumentation, enrichment methods and quantification strategies. In the near future, these technologies will provide a tool that can be used for quantitative investigation of signal transduction pathways to generate new insights into biologic systems.

引用

收藏

页码：343 / 354

页数：12

相关论文

共 62 条

[21] Signaling initiated by overexpression of the fibroblast growth factor receptor-1 investigated by mass spectrometry [J].

Hinsby, AM ;

Olsen, JV ;

Bennettt, KL ;

Mann, M .

MOLECULAR & CELLULAR PROTEOMICS, 2003, 2 (01) :29-36

[22] TRANSFORMING GENE-PRODUCT OF ROUS-SARCOMA VIRUS PHOSPHORYLATES TYROSINE [J].

HUNTER, T ;

SEFTON, BM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (03) :1311-1315

[23] A novel proteomic approach for specific identification of tyrosine kinase substrates using [13C]tyrosine [J].

Ibarrola, N ;

Molina, H ;

Iwahori, A ;

Pandey, A .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (16) :15805-15813

[24] A proteomic approach for quantitation of phosphorylation using stable isotope labeling in cell culture [J].

Ibarrola, N ;

Kalume, DE ;

Gronborg, M ;

Iwahori, A ;

Pandey, A .

ANALYTICAL CHEMISTRY, 2003, 75 (22) :6043-6049

[25] Characterization of serine and threonine phosphorylation sites in β-elimination ethanethiol addition-modified proteins by electrospray tandem mass spectrometry and database searching [J].

Jaffe, H ;

Veeranna ;

Pant, HC .

BIOCHEMISTRY, 1998, 37 (46) :16211-16224

[26] Localization of labile posttranslational modifications by electron capture dissociation:: The case of γ-carboxyglutamic acid [J].

Kelleher, RL ;

Zubarev, RA ;

Bush, K ;

Furie, B ;

Furie, BC ;

McLafferty, FW ;

Walsh, CT .

ANALYTICAL CHEMISTRY, 1999, 71 (19) :4250-4253

[27] CHEMICAL DERIVATIZATION FOR MASS-SPECTROMETRY [J].

KNAPP, DR .

METHODS IN ENZYMOLOGY, 1990, 193 :314-329

[28] Phosphospecific proteolysis for mapping sites of protein phosphorylation [J].

Knight, ZA ;

Schilling, B ;

Row, RH ;

Kenski, DM ;

Gibson, BW ;

Shokat, KM .

NATURE BIOTECHNOLOGY, 2003, 21 (09) :1047-1054

[29] Activating mutations in the epidermal growth factor receptor underlying responsiveness of non-small-cell lung cancer to gefitinib [J].

Lynch, TJ ;

Bell, DW ;

Sordella, R ;

Gurubhagavatula, S ;

Okimoto, RA ;

Brannigan, BW ;

Harris, PL ;

Haserlat, SM ;

Supko, JG ;

Haluska, FG ;

Louis, DN ;

Christiani, DC ;

Settleman, J ;

Haber, DA .

NEW ENGLAND JOURNAL OF MEDICINE, 2004, 350 (21) :2129-2139

[30] Molecular-level description of proteins from Saccharomyces cerevisiae using quadrupole FT hybrid mass spectrometry for top down proteomics [J].

Meng, FY ;

Du, Y ;

Miller, LM ;

Patrie, SM ;

Robinson, DE ;

Kelleher, NL .

ANALYTICAL CHEMISTRY, 2004, 76 (10) :2852-2858

← 1 2 3 4 5 6 7 →